Department of Traditional Chinese Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
J Cell Biochem. 2019 Mar;120(3):3082-3090. doi: 10.1002/jcb.27483. Epub 2018 Nov 26.
Traditional Chinese medicine (TCM) is an effective management to infertility. The association between TCM-mediated fertility and inhibition of phosphatidylinositol-3-kinase (PI3K) would be investigated.
Institute of Cancer Research mice were treated with three herbal decoctions, named Wenshen Yangxue formula, Wenshen formula, and Yangxue formula, plus with human gonadotropins. PI3K inhibitor wortmannin was administrated to half of mice. Some index such as body weight, fertility ability would be investigated. The expression of P13K/Akt signaling was detected by using Western blot analysis.
No difference was observed in body weight among groups. Mice receiving the administration of human gonadotropins and herbal decoctions showed increased follicle numbers, percentage of fertilization, and promoted embryonic development. The treatment of Wenshen Yangxue formula decoction showed the highest efficiency, significant higher than Wenshen and Yangxue formulas. And increased the expression of p-PI3K and p-Akt proteins.
These results suggested the herbal decoctions promoted the fertilization of mice, which was related to the charge of PI3K/Akt activation.
中医（TCM）是一种有效的不孕不育治疗方法。本研究旨在探究 TCM 介导的生育能力与磷脂酰肌醇 3-激酶（PI3K）抑制之间的关联。
将 Institute of Cancer Research 小鼠用三种草药方剂（分别命名为温肾养血方、温肾方和养血方）加人绒毛膜促性腺激素进行处理，一半的小鼠给予 PI3K 抑制剂渥曼青霉素进行处理。检测各组小鼠的体重、生育能力等指标，采用 Western blot 分析检测 PI3K/Akt 信号通路的表达情况。
各组小鼠体重无差异。接受人绒毛膜促性腺激素和草药方剂治疗的小鼠卵泡数量、受精率和胚胎发育均增加。温肾养血方的治疗效果最高，显著高于温肾方和养血方。并增加了 p-PI3K 和 p-Akt 蛋白的表达。
这些结果表明，草药方剂促进了小鼠的受精，这与 PI3K/Akt 激活的作用有关。
