• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CXCR2 在 AC-PGP 诱导的循环血管生成细胞动员中的作用及其治疗意义。

Role of CXCR2 in the Ac-PGP-Induced Mobilization of Circulating Angiogenic Cells and its Therapeutic Implications.

机构信息

Department of Physiology, Pusan National University School of Medicine, Yangsan, Republic of Korea.

Department of Orthopaedic Surgery, Pusan National University School of Medicine, Yangsan, Republic of Korea.

出版信息

Stem Cells Transl Med. 2019 Mar;8(3):236-246. doi: 10.1002/sctm.18-0035. Epub 2018 Nov 26.

DOI:10.1002/sctm.18-0035
PMID:30474937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6392381/
Abstract

Circulating angiogenic cells (CACs) have been implicated in the repair of ischemic tissues, and their mobilization from bone marrow is known to be regulated by the activations of chemokine receptors, including CXCR2 and CXCR4. This study was conducted to investigate the role of N-acetylated proline-glycine-proline (Ac-PGP; a collagen-derived chemotactic tripeptide) on CAC mobilization and its therapeutic potential for the treatment of peripheral artery diseases. Ac-PGP was administered daily to a murine hind limb ischemia model, and the effects of Ac-PGP on blood perfusion and CAC mobilization (Sca1 Flk1 cells) into peripheral blood were assessed. Intramuscular administration of Ac-PGP significantly improved ischemic limb perfusion and increased limb salvage rate by increasing blood vessel formation, whereas Ac-PGP-induced blood perfusion and angiogenesis in ischemic limbs were not observed in CXCR2-knockout mice. In addition, Ac-PGP-induced CAC mobilization was found to occur in wild-type mice but not in CXCR2-knockout mice. Transplantation of bone marrow from green fluorescent protein (GFP) transgenic mice to wild-type mice showed bone marrow-derived cells homed to ischemic limbs after Ac-PGP administration and that GFP-positive cells contributed to the formation of ILB4-positive capillaries and α smooth muscle actin (α-SMA)-positive arteries. These results suggest CXCR2 activation in bone marrow after Ac-PGP administration improves blood perfusion and reduces tissue necrosis by inducing CAC mobilization. These findings suggest a new pharmaceutical basis for the treatment of critical limb ischemia. Stem Cells Translational Medicine 2019;8:236&246.

摘要

循环血管生成细胞（CACs）被认为参与了缺血组织的修复，其从骨髓中的动员被认为是由趋化因子受体（包括 CXCR2 和 CXCR4）的激活所调节的。本研究旨在探讨 N-乙酰化脯氨酸-甘氨酸-脯氨酸（Ac-PGP；一种源自胶原蛋白的趋化三肽）对 CAC 动员的作用及其在治疗外周动脉疾病中的治疗潜力。每天给小鼠后肢缺血模型给予 Ac-PGP，并评估 Ac-PGP 对血液灌注和 CAC 动员（Sca1 Flk1 细胞）进入外周血的影响。肌肉内给予 Ac-PGP 可显著改善缺血肢体的灌注，并通过增加血管形成提高肢体挽救率，而在 CXCR2 敲除小鼠中未观察到 Ac-PGP 诱导的缺血肢体的血液灌注和血管生成。此外，发现 Ac-PGP 诱导的 CAC 动员仅发生在野生型小鼠中，而不在 CXCR2 敲除小鼠中发生。将绿色荧光蛋白（GFP）转基因小鼠的骨髓移植到野生型小鼠中，显示在给予 Ac-PGP 后，骨髓源性细胞归巢到缺血肢体，并且 GFP 阳性细胞有助于形成 ILB4 阳性毛细血管和α平滑肌肌动蛋白（α-SMA）阳性动脉。这些结果表明，Ac-PGP 给药后骨髓中 CXCR2 的激活通过诱导 CAC 动员来改善血液灌注和减少组织坏死。这些发现为治疗严重肢体缺血提供了新的药物治疗基础。《干细胞转化医学》2019 年；8:236&246。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/9bc50968f436/SCT3-8-236-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/d4f8323cbb2c/SCT3-8-236-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/267bd84ccbfe/SCT3-8-236-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/0f7dde5829d4/SCT3-8-236-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/19773ac98455/SCT3-8-236-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/4250046a2587/SCT3-8-236-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/9bc50968f436/SCT3-8-236-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/d4f8323cbb2c/SCT3-8-236-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/267bd84ccbfe/SCT3-8-236-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/0f7dde5829d4/SCT3-8-236-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/19773ac98455/SCT3-8-236-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/4250046a2587/SCT3-8-236-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f2/6392381/9bc50968f436/SCT3-8-236-g006.jpg

相似文献

1
Role of CXCR2 in the Ac-PGP-Induced Mobilization of Circulating Angiogenic Cells and its Therapeutic Implications.CXCR2 在 AC-PGP 诱导的循环血管生成细胞动员中的作用及其治疗意义。
Stem Cells Transl Med. 2019 Mar;8(3):236-246. doi: 10.1002/sctm.18-0035. Epub 2018 Nov 26.
2
N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells.N-乙酰脯氨酸-甘氨酸-脯氨酸通过人内皮祖细胞加速皮肤伤口愈合和血管新生。
Sci Rep. 2017 Feb 23;7:43057. doi: 10.1038/srep43057.
3
Activation of CXCR2 by extracellular matrix degradation product acetylated Pro-Gly-Pro has therapeutic effects against sepsis.细胞外基质降解产物乙酰化 Pro-Gly-Pro 通过激活 CXCR2 对败血症具有治疗作用。
Am J Respir Crit Care Med. 2011 Jul 15;184(2):243-51. doi: 10.1164/rccm.201101-0004OC. Epub 2011 Apr 21.
4
CXCR2 antagonists block the N-Ac-PGP-induced neutrophil influx in the airways of mice, but not the production of the chemokine CXCL1.CXCR2 拮抗剂可阻断 N-Ac-PGP 诱导的小鼠气道中中性粒细胞的浸润，但不能阻断趋化因子 CXCL1 的产生。
Eur J Pharmacol. 2011 Oct 15;668(3):443-9. doi: 10.1016/j.ejphar.2011.03.025. Epub 2011 Mar 31.
5
Angiogenic properties of sustained release platelet-rich plasma: characterization in-vitro and in the ischemic hind limb of the mouse.缓释富血小板血浆的血管生成特性：在小鼠体外和缺血后肢的特性研究
J Vasc Surg. 2009 Oct;50(4):870-879.e2. doi: 10.1016/j.jvs.2009.06.016. Epub 2009 Aug 12.
6
Formyl Peptide Receptor 2 Is Involved in Cardiac Repair After Myocardial Infarction Through Mobilization of Circulating Angiogenic Cells.甲酰肽受体2通过动员循环血管生成细胞参与心肌梗死后的心脏修复。
Stem Cells. 2017 Mar;35(3):654-665. doi: 10.1002/stem.2535. Epub 2016 Nov 25.
7
Estradiol-induced, endothelial progenitor cell-mediated neovascularization in male mice with hind-limb ischemia.雌二醇诱导的、内皮祖细胞介导的雄性后肢缺血小鼠血管新生
Vasc Med. 2009 Feb;14(1):29-36. doi: 10.1177/1358863X08096666.
8
Deletion of FHL2 gene impaired ischemia-induced blood flow recovery by modulating circulating proangiogenic cells.FHL2 基因缺失通过调节循环促血管生成细胞损害缺血诱导的血流恢复。
Arterioscler Thromb Vasc Biol. 2013 Apr;33(4):709-17. doi: 10.1161/ATVBAHA.112.300318. Epub 2013 Feb 14.
9
N-acetylated Proline-Glycine-Proline induced G-protein dependent chemotaxis of neutrophils is independent of CXCL8 release.N-乙酰化脯氨酸-甘氨酸-脯氨酸诱导的中性粒细胞 G 蛋白依赖性趋化作用不依赖于 CXCL8 的释放。
Eur J Pharmacol. 2011 Oct 15;668(3):428-34. doi: 10.1016/j.ejphar.2011.03.022. Epub 2011 Mar 31.
10
Collagen-Derived N-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype.椎间盘胶原衍生的 N-乙酰化脯氨酰-甘氨酰-脯氨酸调节软骨终板干细胞中 CXCR1/2 的表达和激活，诱导其向促炎表型迁移和分化。
Stem Cells. 2015 Dec;33(12):3558-68. doi: 10.1002/stem.2200.

引用本文的文献

1
GC-MS based comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis.基于 GC-MS 的人松质骨比较代谢组学分析揭示了亚油酸代谢在股骨头坏死中的关键作用。
Metabolomics. 2023 Sep 30;19(10):86. doi: 10.1007/s11306-023-02053-3.
2
Increasing the Therapeutic Potential of Stem Cell Therapies for Critical Limb Ischemia.提高干细胞疗法对严重肢体缺血的治疗潜力。
HSOA J Stem Cells Res Dev Ther. 2020;6(1). doi: 10.24966/srdt-2060/100024. Epub 2020 Jan 20.
3
Application of periostin peptide-decorated self-assembled protein cage nanoparticles for therapeutic angiogenesis.

本文引用的文献

1
N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells.N-乙酰脯氨酸-甘氨酸-脯氨酸通过人内皮祖细胞加速皮肤伤口愈合和血管新生。
Sci Rep. 2017 Feb 23;7:43057. doi: 10.1038/srep43057.
2
Formyl Peptide Receptor 2 Is Involved in Cardiac Repair After Myocardial Infarction Through Mobilization of Circulating Angiogenic Cells.甲酰肽受体2通过动员循环血管生成细胞参与心肌梗死后的心脏修复。
Stem Cells. 2017 Mar;35(3):654-665. doi: 10.1002/stem.2535. Epub 2016 Nov 25.
3
Matrix-derived inflammatory mediator N-acetyl proline-glycine-proline is neurotoxic and upregulated in brain after ischemic stroke.
外泌体载姜黄素前体脂质体凝胶的制备及其体内评价
BMB Rep. 2022 Apr;55(4):175-180. doi: 10.5483/BMBRep.2022.55.4.137.
4
Combined administration of laminin-221 and prostacyclin agonist enhances endogenous cardiac repair in an acute infarct rat heart.层粘连蛋白-221 与前列环素激动剂联合给药增强急性心肌梗死大鼠心脏内源性修复。
Sci Rep. 2021 Nov 15;11(1):22243. doi: 10.1038/s41598-021-00918-y.
基质衍生的炎症介质 N-乙酰脯氨酸-甘氨酸-脯氨酸具有神经毒性，并且在缺血性中风后脑中上调。
J Neuroinflammation. 2015 Nov 21;12:214. doi: 10.1186/s12974-015-0428-z.
4
Extrarenal Progenitor Cells Do Not Contribute to Renal Endothelial Repair.肾外祖细胞对肾内皮修复无贡献。
J Am Soc Nephrol. 2016 Jun;27(6):1714-26. doi: 10.1681/ASN.2015030321. Epub 2015 Oct 9.
5
The real face of endothelial progenitor cells - Circulating angiogenic cells as endothelial prognostic marker?内皮祖细胞的真面目——循环血管生成细胞作为内皮预后标志物？
Pharmacol Rep. 2015 Aug;67(4):793-802. doi: 10.1016/j.pharep.2015.05.017. Epub 2015 Jun 11.
6
Collagen-Derived N-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype.椎间盘胶原衍生的 N-乙酰化脯氨酰-甘氨酰-脯氨酸调节软骨终板干细胞中 CXCR1/2 的表达和激活，诱导其向促炎表型迁移和分化。
Stem Cells. 2015 Dec;33(12):3558-68. doi: 10.1002/stem.2200.
7
Mobilization of sca1/flk-1 positive endothelial progenitor cells declines in apolipoprotein E-deficient mice with a high-fat diet.在高脂饮食的载脂蛋白E缺陷小鼠中，sca1/flk-1阳性内皮祖细胞的动员能力下降。
J Cardiol. 2015 Dec;66(6):532-8. doi: 10.1016/j.jjcc.2015.02.008. Epub 2015 Mar 24.
8
A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.一种新型分子Me6TREN通过增强内皮祖细胞的动员和募集来促进血管生成。
Sci Rep. 2014 Aug 28;4:6222. doi: 10.1038/srep06222.
9
WKYMVm-induced activation of formyl peptide receptor 2 stimulates ischemic neovasculogenesis by promoting homing of endothelial colony-forming cells.WKYMVm 诱导的甲酰肽受体 2 激活通过促进内皮祖细胞归巢来刺激缺血性新血管生成。
Stem Cells. 2014 Mar;32(3):779-90. doi: 10.1002/stem.1578.
10
Circulating and tissue resident endothelial progenitor cells.循环和组织驻留内皮祖细胞。
J Cell Physiol. 2014 Jan;229(1):10-6. doi: 10.1002/jcp.24423.