Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Chongqing Medical University, Chongqing, 400016, China.
Metabolomics. 2023 Sep 30;19(10):86. doi: 10.1007/s11306-023-02053-3.
Femur head necrosis (FHN) is a challenging clinical disease with unclear underlying mechanism, which pathologically is associated with disordered metabolism. However, the disordered metabolism in cancellous bone of FHN was never analyzed by gas chromatography-mass spectrometry (GC-MS).
To elucidate altered metabolism pathways in FHN and identify putative biomarkers for the detection of FHN.
We recruited 26 patients with femur head necrosis and 22 patients with femur neck fracture in this study. Cancellous bone tissues from the femoral heads were collected after the surgery and were analyzed by GC-MS based untargeted metabolomics approach. The resulting data were analyzed via uni- and multivariate statistical approaches. The changed metabolites were used for the pathway analysis and potential biomarker identification.
Thirty-seven metabolites distinctly changed in FHN group were identified. Among them, 32 metabolites were upregulated and 5 were downregulated in FHN. The pathway analysis showed that linoleic acid metabolism were the most relevant to FHN pathology. On the basis of metabolites network, L-lysine, L-glutamine and L-serine were deemed as the junctions of the whole metabolites. Finally, 9,12-octadecadienoic acid, inosine, L-proline and octadecanoic acid were considered as the potential biomarkers of FHN.
This study provides a new insight into the pathogenesis of FHN and confirms linoleic acid metabolism as the core.
股骨头坏死（FHN）是一种具有不明潜在机制的具有挑战性的临床疾病，其病理上与代谢紊乱有关。然而，FHN 松质骨中的代谢紊乱从未通过气相色谱-质谱（GC-MS）进行分析。
阐明 FHN 中的代谢紊乱途径，并确定用于检测 FHN 的潜在生物标志物。
本研究纳入了 26 例股骨头坏死患者和 22 例股骨颈骨折患者。手术切除股骨头后采集松质骨组织，采用基于 GC-MS 的非靶向代谢组学方法进行分析。通过单变量和多变量统计方法对所得数据进行分析。利用改变的代谢物进行途径分析和潜在生物标志物的鉴定。
在 FHN 组中鉴定出 37 种明显改变的代谢物。其中，FHN 组有 32 种代谢物上调，5 种代谢物下调。途径分析表明，亚油酸代谢与 FHN 病理最相关。基于代谢物网络，L-赖氨酸、L-谷氨酰胺和 L-丝氨酸被认为是整个代谢物的交汇点。最后，9,12-十八碳二烯酸、肌苷、L-脯氨酸和十八烷酸被认为是 FHN 的潜在生物标志物。
本研究为 FHN 的发病机制提供了新的见解，并证实亚油酸代谢是核心。
