Wang Yan-Hong, Kumarihamy Mallika, Wang Mei, Smesler Andrew, Khan Ikhlas A, León Francisco, Cutler Stephen J, Muhammad Ilias
Nat Prod Commun. 2016 Nov;11(11):1689-1692.
A simple, rapid, and sensitive UHPLC-UV-MS method was developed for the quantitative determination of betaine (1), choline (2), acetylcholine (3), and 20-hydroxyecdysone (4) from various species of Atriplex. The baseline separation of the four analytes was achieved on a reversed phase C 18 column within nine minutes. The mobile phase was composed of 50 mM ammonium formate in 2% methanol-water containing 5 mM sodium dodecyl sulfate (pH = 8.2) and methanol with 0.01% ammonium hydroxide. The analytical method was validated for recovery, precision, limits of detection (LOD), and limits of quantification (LOQ). The developed method was applied for the characterization and quantitation of analytes from plant parts of different Atriplex species, including A. canescens, A. fruticulosa, A. fasciculata, A. semibaccata, and A. lentiformis. Compounds 1-4 were found in a range of 0.53-1.61%, detection under limit of quantification (DUL)-0.74, DUL-0.0038, and 0-0.10% (w/w, mg in 100 mg plant material), respectively, in test samples. In leaf and fruit of A. canescens, a high content of 1, 2, and 4 were identified. The content of 1, 2, and 4 in A. canescens explains the potential implications of this native US plant for human health and nutrition. The result of this study provides a new method to analyze these phytoconstituents simultaneously in a mixture.
建立了一种简单、快速、灵敏的超高效液相色谱-紫外-质谱法，用于定量测定不同滨藜属植物中的甜菜碱（1）、胆碱（2）、乙酰胆碱（3）和20-羟基蜕皮甾酮（4）。在反相C18柱上9分钟内实现了四种分析物的基线分离。流动相由含5 mM十二烷基硫酸钠（pH = 8.2）的2%甲醇-水和含0.01%氢氧化铵的甲醇中的50 mM甲酸铵组成。该分析方法在回收率、精密度、检测限（LOD）和定量限（LOQ）方面得到了验证。所建立的方法用于不同滨藜属植物（包括灰绿滨藜、灌木滨藜、束滨藜、半浆果滨藜和透镜状滨藜）植物部位中分析物的表征和定量。在测试样品中，化合物1-4的含量分别在0.53-1.61%、低于定量限检测（DUL）-0.74、DUL-0.0038和0-0.10%（w/w，100 mg植物材料中的毫克数）范围内。在灰绿滨藜的叶和果实中，鉴定出高含量的1、2和4。灰绿滨藜中1、2和4的含量解释了这种美国本土植物对人类健康和营养的潜在影响。本研究结果提供了一种同时分析混合物中这些植物成分的新方法。
