College of Agriculture, Northeast Agricultural University, Harbin, 150030, China.
College of Agriculture, Northeast Agricultural University, Harbin, 150030, China.
Comput Biol Chem. 2018 Dec;77:413-429. doi: 10.1016/j.compbiolchem.2018.09.012. Epub 2018 Oct 5.
Heat and drought are the two major abiotic stress limiting soybean growth and output worldwide. Knowledge of the molecular mechanisms underlying the responses to heat, drought, and combined stress is essential for soybean molecular breeding. In this study, RNA-sequencing was used to determine the transcriptional responses of soybean to heat, drought and combined stress. RNA-sequencing analysis demonstrated that many genes involved in the defense response, photosynthesis, metabolic process, etc. are differentially expressed in response to drought and heat. However, 1468 and 1220 up-regulated and 1146 and 686 down-regulated genes were confirmed as overlapping differentially expressed genes at 8 h and 24 h after treatment, and these genes are mainly involved in transport, binding and defense response. Furthermore, we compared the heat, drought and the combined stress-responsive genes and identified potential new targets for enhancing stress tolerance of soybean. Comparison of single and combined stress suggests the combined stress did not result in a simple additive response, and that there may be a synergistic response to the combination of drought and heat in soybean.
热胁迫和干旱胁迫是全球范围内限制大豆生长和产量的两种主要非生物胁迫。了解大豆对热胁迫、干旱胁迫和复合胁迫响应的分子机制对于大豆分子育种至关重要。本研究利用 RNA 测序技术研究了大豆对热胁迫、干旱胁迫和复合胁迫的转录组响应。RNA 测序分析表明，许多参与防御反应、光合作用、代谢过程等的基因在应对干旱和热胁迫时表现出差异表达。然而，在处理后 8 小时和 24 小时，分别有 1468 个和 1220 个上调基因和 1146 个和 686 个下调基因被确定为重叠差异表达基因，这些基因主要参与运输、结合和防御反应。此外，我们比较了热胁迫、干旱胁迫和复合胁迫响应基因，鉴定了增强大豆胁迫耐受性的潜在新靶点。单一胁迫和复合胁迫的比较表明，复合胁迫没有导致简单的累加响应，并且在大豆中干旱和热胁迫的组合可能存在协同响应。
