Chen Shengyan, Chen Bin, Xu Xingnong
College of Landscape Architecture, Northeast Forestry University, Haerbin, Heilongjiang, 150040, China.
Yancheng Third People's Hospital, Yancheng Jiangsu, the affiliated hospital of Jiangsu Vocational College of Medicine, Yancheng, Jiangsu, 224008, China.
BMC Plant Biol. 2025 May 13;25(1):631. doi: 10.1186/s12870-025-06521-x.
Chrysanthemum indicum is an important medicinal plant that has a particularly strong effect on bacteria and viruses and has antioxidant, anti-inflammatory, and immunomodulatory properties. The genes of the TCP family, a group of plant-specific transcription factors (TFs), have been found to play a crucial role in the regulation of plant growth and development as well as resistance to abiotic stress. Nevertheless, no systematic analysis of the TCP family genes in C. indicum has been performed so far. In the present study, a total of 26 non-redundant CiTCP genes were identified in the genome of C. indicum. The TCP genes were categorized into three subgroups on the basis of the phylogenetic analysis: 7, 9, and 10 genes belonged to the CIN subgroup, CYC/TB1 subgroup, and PCF subgroup, respectively. All CiTCPs were unevenly distributed across the 9 chromosomes. TCP genes in the same subgroup showed similar gene structures and conserved motifs. Gene duplication analysis revealed that segmental duplications had a significant effect on the expansion of CiTCP genes. The analysis of cis-elements revealed that CiTCP genes may be involved in the regulation of plant development, hormone response and response to abiotic stress. Expression profile analysis of the transcriptome data indicated that CiTCP genes exhibited similar or distinct expressions within different tissues and under different abiotic stresses. According to the results of quantitative RT-PCR (qRT-PCR), the expression of 15 selected genes responded strongly to various abiotic stress factors. The results of our studies could provide comprehensive insights into the TCP family genes of C. indicum for further functional investigations.
野菊花是一种重要的药用植物，对细菌和病毒有特别强的作用，具有抗氧化、抗炎和免疫调节特性。TCP家族的基因是一组植物特异性转录因子（TFs），已发现它们在植物生长发育调控以及对非生物胁迫的抗性中起关键作用。然而，迄今为止尚未对野菊花中的TCP家族基因进行系统分析。在本研究中，在野菊花基因组中总共鉴定出26个非冗余的CiTCP基因。基于系统发育分析，TCP基因被分为三个亚组：分别有7、9和10个基因属于CIN亚组、CYC/TB1亚组和PCF亚组。所有CiTCP基因不均匀地分布在9条染色体上。同一亚组中的TCP基因显示出相似的基因结构和保守基序。基因复制分析表明，片段重复对CiTCP基因的扩增有显著影响。顺式元件分析表明，CiTCP基因可能参与植物发育、激素应答和对非生物胁迫的应答调控。转录组数据的表达谱分析表明，CiTCP基因在不同组织和不同非生物胁迫下表现出相似或不同的表达。根据定量RT-PCR（qRT-PCR）结果，15个选定基因的表达对各种非生物胁迫因子有强烈响应。我们的研究结果可为进一步功能研究提供对野菊花TCP家族基因的全面见解。
