Yu K L, Sherwood N M, Peter R E
Department of Zoology, University of Alberta, Edmonton, Canada.
Peptides. 1988 May-Jun;9(3):625-30. doi: 10.1016/0196-9781(88)90174-x.
Two molecular forms of gonadotropin-releasing hormone (GnRH) were identified in the extracts of various brain areas, spinal cord and pituitary in female and male goldfish and had chromatographic and immunological properties similar to [His5, Trp7, Tyr8]-GnRH (cGnRH-II) and [Trp7,Leu8]-GnRH (sGnRH). Radioimmunoassay using different GnRH antisera after high pressure liquid chromatography did not reveal significant peaks of mammalian GnRH, [Gln8]-GnRH and [Tyr3,Leu5,Glu6,Trp7,Lys8]-GnRH in the brain extracts. The proportion of cGnRH-II-like immunoactivity to sGnRH-like immunoactivity was higher in the caudal brain areas compared to the rostral areas. The differential distribution of two GnRH forms suggest that the different GnRH forms may have different physiological functions.
在雌性和雄性金鱼的各个脑区、脊髓和垂体提取物中鉴定出两种促性腺激素释放激素（GnRH）分子形式，它们具有与[His5, Trp7, Tyr8]-GnRH（cGnRH-II）和[Trp7,Leu8]-GnRH（sGnRH）相似的色谱和免疫特性。高压液相色谱后使用不同的GnRH抗血清进行放射免疫测定，未在脑提取物中发现哺乳动物GnRH、[Gln8]-GnRH和[Tyr3,Leu5,Glu6,Trp7,Lys8]-GnRH的显著峰。与脑前部区域相比，cGnRH-II样免疫活性与sGnRH样免疫活性的比例在脑后部区域更高。两种GnRH形式的差异分布表明，不同的GnRH形式可能具有不同的生理功能。
