通过调控 NF-κB 和 p38/JNK 通路发挥抗炎作用。

Anti-Inflammatory Activity of Leaf Extract via Modulating NF-κB and p38/JNK Pathways.

机构信息

Department of Medical Science, College of Medical Science, Soonchunhyang University, Asan, Chungnam 31538, Korea.

Department of Medical Biotechnology, College of Medical Science, Soonchunhyang University, Asan, Chungnam 31538, Korea.

出版信息

Int J Mol Sci. 2018 Nov 25;19(12):3746. doi: 10.3390/ijms19123746.

, known as eastern cottonwood, has been commonly used as a medicinal plant. The aim of the present study was to investigate the mechanism underlying the anti-inflammatory activity of leaf extract (PLE). PLE effectively inhibited the expression of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, but not that of cyclooxygenase-2 (COX-2) and prostaglandin E2. Proinflammatory tumor necrosis factor alpha (TNF-α) levels were also reduced by the extract. PLE inhibited the phosphorylation of nuclear factor-kappa B (NF-κB) and inhibitor of Kappa Bα (IκBα), and blunted LPS-triggered enhanced nuclear translocation of NF-κB p65. In mitogen-activated protein kinase (MAPK) signaling, PLE effectively decreased the phosphorylation of p38 and c-Jun N-terminal protein kinase (JNK), but not of extracellular signal-regulated kinase 1/2 (ERK1/2). Taken together, these results suggest that anti-inflammatory activity of leaf extract might be driven by iNOS and NO inhibition mediated by modulation of the NF-κB and p38/JNK signaling pathways.

，又名东方棉白杨，常被用作药用植物。本研究旨在探讨叶提取物（PLE）抗炎活性的作用机制。PLE 可有效抑制脂多糖（LPS）刺激的 RAW 264.7 细胞中诱导型一氧化氮合酶（iNOS）的表达和一氧化氮（NO）的产生，但不抑制环氧化酶-2（COX-2）和前列腺素 E2 的表达。该提取物还能降低促炎肿瘤坏死因子-α（TNF-α）的水平。PLE 抑制核因子-κB（NF-κB）和κB 抑制蛋白α（IκBα）的磷酸化，并减弱 LPS 触发的 NF-κB p65 的核易位增强。在丝裂原活化蛋白激酶（MAPK）信号通路中，PLE 能有效降低 p38 和 c-Jun N 端蛋白激酶（JNK）的磷酸化，但不影响细胞外信号调节激酶 1/2（ERK1/2）的磷酸化。综上所述，这些结果表明，叶提取物的抗炎活性可能是通过调节 NF-κB 和 p38/JNK 信号通路来抑制 iNOS 和 NO 介导的。