Fan Ruyan, Fan Chuping, Zhang Jian, Wen Bo, Lei Yefei, Liu Chan, Chen Lijuan, Liu Wenpei, Wang Chuan, Qu Xiaowang
Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
Department of Pediatric, Guangzhou Women and Children's Medical Center, Guangzhou, People's Republic of China.
J Med Virol. 2017 Feb;89(2):213-221. doi: 10.1002/jmv.24619. Epub 2016 Jul 6.
Respiratory syncytial virus (RSV) infection is the leading cause of acute respiratory tract disease in children less than 5 years old. The aim of this study was to further elucidate the molecular properties and clinical characteristics of RSV infection. The study sample included 238 patients <5 years old who were hospitalized with clinical symptoms of upper or lower respiratory tract infection (URTI or LRTI) in the Pediatric Department at the First People's Hospital of Chenzhou, South China in 2014. We subjected nasopharyngeal aspirate (NPA) or nasal swab (NS) samples from the patients to indirect fluorescence assay screens. RSV G genes were amplified by reverse transcription-PCR (RT-PCR) and sequenced. Of the 238 patients screened, 64 (26.8%) were confirmed to have RSV infections. Of those 64 confirmed RSV infection cases, 39 (60.9%) had subtype BA9, 13 (20.3%) had the recently identified subtype ON1, 11 (17.2%) had subtype NA1, and 1 (1.6%) had subtype GB2. The predominant presentation was LRTI with coughing, sputum production, fever, and wheezing. RSV subtype NA1 and BA9 infections were found mostly in infants, whereas the age distribution of subtype ON1 infections was more uniform across the age bands. Phylogenetic analysis indicated that, compared with the prototype strain A2, all ON1 and most NA1 isolates had lost one potential N-glycosylation site at amino acid 251 and 249 due to T251K and N249Y substitution, respectively. These findings suggest that NA1, BA9, and ON1 are the dominant RSV subtypes causing respiratory tract infections in young children presenting to the hospital in South China. J. Med. Virol. 89:213-221, 2017. © 2016 Wiley Periodicals, Inc.
呼吸道合胞病毒（RSV）感染是5岁以下儿童急性呼吸道疾病的主要病因。本研究的目的是进一步阐明RSV感染的分子特性和临床特征。研究样本包括2014年在中国南方郴州市第一人民医院儿科因上呼吸道或下呼吸道感染（URTI或LRTI）临床症状住院的238例5岁以下患者。我们对患者的鼻咽抽吸物（NPA）或鼻拭子（NS）样本进行间接荧光检测筛选。通过逆转录聚合酶链反应（RT-PCR）扩增RSV G基因并进行测序。在筛选的238例患者中，64例（26.8%）被确诊为RSV感染。在这64例确诊的RSV感染病例中，39例（60.9%）为BA9亚型，13例（20.3%）为最近鉴定出的ON1亚型，11例（17.2%）为NA1亚型，1例（1.6%）为GB2亚型。主要表现为LRTI，伴有咳嗽、咳痰、发热和喘息。RSV NA1和BA9亚型感染多见于婴儿，而ON1亚型感染的年龄分布在各年龄组更为均匀。系统发育分析表明，与原型株A2相比，所有ON1和大多数NA1分离株分别由于T251K和N249Y取代，在氨基酸251和249处失去了一个潜在的N-糖基化位点。这些发现表明，NA1、BA9和ON1是导致中国南方医院就诊幼儿呼吸道感染的主要RSV亚型。《医学病毒学杂志》89:213 - 221，2017年。©2016威利期刊公司
