Del Mundo Crisfel R, Castillo Agnes L, An Seong Soo A, Tan Mario A
1The Graduate School, University of Santo Tomas, Manila, Philippines.
5School of Pharmacy, Centro Escolar University, Manila, Philippines.
3 Biotech. 2020 Mar;10(3):90. doi: 10.1007/s13205-020-2087-8. Epub 2020 Feb 4.
The methanolic (PTM) and aqueous (PTA) extracts were investigated for their potential in vivo anti-inflammatory activity using carrageenan-induced rat paw edema. Parameters including paw thickness measurement, histopathological, and immunohistochemical cyclooxygenase (COX)-2 expression analyses were measured and analyzed. PTA at 500 mg/kg significantly reduced inflammation after induction of carrageenan (Carr), with mean paw thickness change of 0.110 ± 0.024 mm at sixth hour post-induction and histopathological mean inflammatory grade of 1.80 ± 0.20. The reduced immunohistochemical COX-2 expression using PTA at 500 mg/kg was determined with mean final 3,3'-diaminobenzidine (DAB) intensity of 63.70 ± 2.08. The profiling of metabolites by liquid chromatography-mass spectrometry (LC-MS) revealed presence of ethyl caffeate and dihydroconiferyl alcohol as putative secondary metabolites of PTA which were the major peaks and have been reported to possess anti-inflammatory activities. This research has provided scientific insights of utilizing as a potential anti-inflammatory agent containing secondary metabolites which may be pharmacologically relevant.
使用角叉菜胶诱导的大鼠足爪肿胀模型，研究了甲醇提取物（PTM）和水提取物（PTA）的体内抗炎活性潜力。测量并分析了包括足爪厚度测量、组织病理学以及免疫组织化学环氧化酶（COX）-2表达分析等参数。500mg/kg的PTA在角叉菜胶诱导后显著减轻了炎症，诱导后第六小时足爪平均厚度变化为0.110±0.024mm，组织病理学平均炎症等级为1.80±0.20。使用500mg/kg的PTA使免疫组织化学COX-2表达降低，最终3,3'-二氨基联苯胺（DAB）平均强度为63.70±2.08。通过液相色谱-质谱联用（LC-MS）对代谢物进行分析，结果显示咖啡酸乙酯和二氢松柏醇作为PTA的推定次生代谢物存在，它们是主要峰，并且据报道具有抗炎活性。本研究为将其作为一种含有可能具有药理学相关性的次生代谢物的潜在抗炎剂的利用提供了科学见解。
