Institute for Molecular Medicine, Texas Center for Health Disparities, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76017, USA.
Keck School of Medicine of University of Southern California, 1450 Biggy Street, NRT 2516, Los Angeles, CA, 90089-9601, USA.
Breast Cancer Res Treat. 2019 Feb;174(1):113-120. doi: 10.1007/s10549-018-5030-5. Epub 2018 Nov 26.
Our aim was to determine the role of Annexin A2 (AnxA2), which we have previously found to contribute to the aggressiveness of TNBC, with AA TNBC patients and clinical outcome.
We analyzed TCGA breast cancer database (n = 1098) to observe AnxA2 expression within breast cancer subtypes and is correlation with overall survival. Further, we examined breast tissue specimens (n = 119) through chromogenic in situ hybridization (CISH) and specimen were scored independently by two pathologists in a blinded study.
In our TCGA analysis, high expression of AnxA2 was correlated with poor survival in patients with TNBC. AnxA2 gene expression was not correlated with poor survival in other breast cancer subtypes. AnxA2 average CISH intensity score (CISH score = 0, null expression to 3, high expression) for TNBC was significantly higher in comparison to estrogen receptor and/or progesterone receptor positive, human epidermal growth factor positive, and non-malignant tissues. Furthermore, AnxA2 average score was significantly higher in AA TNBC patients (CISH average score = 2.45 ± 0.3266) in comparison to Caucasian TNBC patients (CISH average score = 1.1 ± 0.4069).
AnxA2 is overexpressed in TNBC, implicating AnxA2 as a contributor to the aggressive biology of TNBC in AA women.
我们的目的是确定膜联蛋白 A2（AnxA2）的作用，我们之前发现它有助于三阴性乳腺癌（TNBC）的侵袭性，分析 AA 型 TNBC 患者与临床结局的关系。
我们分析了 TCGA 乳腺癌数据库（n=1098），观察了乳腺癌亚型中的 AnxA2 表达及其与总生存期的相关性。此外，我们通过显色原位杂交（CISH）检查了 119 例乳腺组织标本，并由两名病理学家在盲法研究中独立评分。
在我们的 TCGA 分析中，AnxA2 高表达与 TNBC 患者的不良预后相关。AnxA2 基因表达与其他乳腺癌亚型的不良预后无关。与雌激素受体和/或孕激素受体阳性、人表皮生长因子阳性和非恶性组织相比，TNBC 的 AnxA2 平均 CISH 强度评分（CISH 评分=0，无表达至 3，高表达）显著更高。此外，与高加索 TNBC 患者（CISH 平均评分=1.1±0.4069）相比，AA 型 TNBC 患者的 AnxA2 平均评分显著更高（CISH 平均评分=2.45±0.3266）。
AnxA2 在 TNBC 中过度表达，提示 AnxA2 是 AA 女性 TNBC 侵袭性生物学的一个贡献因素。
