Hiruta Emika, Aizawa Misato, Nakano Akio, Sonoda Shoji
School of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321-8505, Japan.
Kagawa Agricultural Experiment Station, Ayauta, Kagawa 761-2306, Japan.
J Pestic Sci. 2018 Nov 20;43(4):272-276. doi: 10.1584/jpestics.D18-007.
The flower thrips strain showing resistance to spinosad was established in the laboratory. The resistant strain showed an LC value of 1398.7 mg/L in a leaf dipping/contact assay. The LC value was 280 times higher than that of the most susceptible strain. An insecticidal assay using synergists suggested no involvement of degradation enzymes, such as cytochrome P450, glutathione -transferase, and carboxyl esterase, in the resistance. Glycine at amino acid position 275 of the nicotinic acetylcholine receptor (nAChR) α6 subunit was mutated to valine in the resistant strain. These results suggest that spinosad resistance in is conferred by the reduced sensitivity of nAChR.
在实验室中建立了对多杀菌素具有抗性的西花蓟马品系。在浸叶/接触试验中，该抗性品系的LC值为1398.7 mg/L。该LC值比最敏感品系高280倍。使用增效剂的杀虫试验表明，抗性与细胞色素P450、谷胱甘肽 -转移酶和羧酸酯酶等降解酶无关。在抗性品系中，烟碱型乙酰胆碱受体（nAChR）α6亚基第275位氨基酸的甘氨酸突变为缬氨酸。这些结果表明，nAChR敏感性降低赋予了西花蓟马对多杀菌素的抗性。
