Sun Li-Na, Shen Xiu-Jing, Cao Li-Jun, Chen Jin-Cui, Ma Li-Jun, Wu San-An, Hoffmann Ary Anthony, Wei Shu-Jun
Beijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing 100083, China.
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Insects. 2022 Mar 28;13(4):331. doi: 10.3390/insects13040331.
The western flower thrips (Pergande) (Thysanoptera: Thripidae) is an important invasive pest worldwide. Field-evolved resistance to the pesticide spinetoram is an increasing problem in the chemical control of this pest. Here, we examined changes in the frequency of a genetic mutation associated with spinetoram resistance, the G275E mutation in the acetylcholine receptor , in 62 field populations collected from 2009 to 2021 across areas of China invaded by this pest. We found a low frequency of the G275E mutation in populations collected at the early invasion stage, in contrast to a high frequency in native USA populations. However, the frequency of the G275E mutation has increased to a high level in recently collected populations, with the mutation becoming fixed in some populations. There was a correlation between the frequency of the G275E mutation and resistance to spinetoram as characterized by median lethal concentration, although two populations were outliers. These results showed that G275E mutation is one of the mechanisms conferring spinetoram resistance in many invading populations in China. Ongoing dispersal of the WFT may have facilitated a rapid increase in the G275E mutation across China. Our study highlights the rapid evolution of pesticide resistance in an invasive species and points to a useful marker for molecular diagnostics of spinetoram resistance.
西花蓟马（佩尔甘德）（缨翅目：蓟马科）是一种在全球范围内具有重要影响的入侵害虫。在对该害虫的化学防治中，田间进化出对多杀霉素的抗性是一个日益严重的问题。在此，我们研究了与多杀霉素抗性相关的基因突变频率的变化，即乙酰胆碱受体中的G275E突变，该研究涉及从2009年至2021年在中国该害虫入侵地区采集的62个田间种群。我们发现，在入侵早期阶段采集的种群中，G275E突变的频率较低，而在美国本土种群中该频率较高。然而，在最近采集的种群中，G275E突变的频率已上升至较高水平，在一些种群中该突变已固定下来。尽管有两个种群是异常值，但G275E突变的频率与以半数致死浓度表征的对多杀霉素的抗性之间存在相关性。这些结果表明，G275E突变是中国许多入侵种群中赋予多杀霉素抗性的机制之一。西花蓟马的持续扩散可能促进了G275E突变在中国的快速增加。我们的研究突出了入侵物种中抗药性的快速进化，并指出了一个用于多杀霉素抗性分子诊断的有用标记。
