Rêgo W M F, Costa J G L, Baraviera R C A, Pinto L V, Bessa G L, Lopes R E N, Silveira J A G, Vitor R W A
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (ICB-UFMG), CEP, 31270-901, Belo Horizonte, MG, Brazil.
Int J Parasitol Parasites Wildl. 2018 Nov 10;7(3):432-438. doi: 10.1016/j.ijppaw.2018.11.001. eCollection 2018 Dec.
Recent studies carried out in Brazil have shown that strains from the same genotype can infect humans, domestic animals (dogs and cats) and animals slaughtered for human consumption (pigs, sheep, goats, and chickens), suggesting a common infection route. However, little is known about the importance of free-living wild birds within this epidemiological context. The objective of this work was to isolate, genotype, and evaluate the virulence for mice of new isolates of obtained from free-living wild birds from the state of Minas Gerais, Southeastern Brazil. From August 2016 to June 2017, was isolated from the hearts and brains collected from 6 out of 45 free-living wild birds, namely, a roadside hawk (), a campo flicker (), a southern caracara () and a tropical screech-owl (), all rescued in Belo Horizonte. One isolate was obtained from a toco toucan (), rescued in Cristiano Otoni, and another was obtained from southern caracara, rescued in Santa Luzia. Five different genotypes were identified by PCR-RFLP. A unique genotype was shared in two different isolates obtained from a southern caracara and a toco toucan. This genotype has never been previously described in any other host or place. Three isolates were classified as of intermediary virulence and three isolates as avirulent for mice. The combined analysis of alleles ROP18/ROP5 (a serine/threonine kinase, and a polymorphic pseudokinase, respectively) was effective in determining the virulence of five of all the isolates with the exception of that from . Atypical isolates of obtained from free-living wild birds rescued in the state of Minas Gerais share the same genotypes of strains that infect humans, domestic animals, and animals slaughtered for human consumption.
巴西最近开展的研究表明，相同基因型的菌株可感染人类、家畜（狗和猫）以及供人类食用而屠宰的动物（猪、绵羊、山羊和鸡），这表明存在共同的感染途径。然而，对于自由生活的野生鸟类在这一流行病学背景下的重要性，人们了解甚少。本研究的目的是从巴西东南部米纳斯吉拉斯州自由生活的野生鸟类中分离新的菌株、进行基因分型并评估其对小鼠的毒力。2016年8月至2017年6月，从45只自由生活的野生鸟类中的6只采集的心脏和大脑中分离出了该菌株，这6只鸟分别是在贝洛奥里藏特获救的一只路边鹰、一只野扑翅䴕、一只南方卡拉卡拉鹰和一只热带角鸮，还有一只在克里斯蒂亚诺·奥托尼获救的托哥巨嘴鸟，以及一只在圣卢西亚获救的南方卡拉卡拉鹰。通过PCR-RFLP鉴定出了五种不同的基因型。从一只南方卡拉卡拉鹰和一只托哥巨嘴鸟获得的两个不同分离株共享一种独特的基因型。这种基因型此前从未在任何其他宿主或地点被描述过。三个分离株被分类为对小鼠具有中等毒力，三个分离株对小鼠无毒力。除了来自[具体名称未给出]的分离株外，等位基因ROP18/ROP5（分别为一种丝氨酸/苏氨酸激酶和一种多态性假激酶）的联合分析有效地确定了所有分离株中五个的毒力。从米纳斯吉拉斯州获救的自由生活野生鸟类中获得的该菌的非典型分离株与感染人类、家畜以及供人类食用而屠宰的动物的菌株具有相同的基因型。
Zotero 插件