Knospe W H, Mortenson R, Husseini S, Trobaugh F E
Exp Hematol. 1978 Feb;6(2):233-45.
Cellulose ester membranes (CEM) implanted into the peritoneal cavity of mice rapidly became coated with cells of peritoneal origin. Up to 56% of the cells, at a peak point 3-5 days after implantation, showed cell membrane receptors for complement and cytophilic immunoglobulin. A similar proportion of cells from CEM phagocytized yeast particles in vitro. When studied in situ, rosettes with C3b and IgG coated erythrocytes were formed by 23% of the cells coating CEM. A decreasing percentage of cells with monocyte-macrophage characteristics were detected between 5 and 17 days. CEM removed at 2 or 6 weeks after peritoneal implantation enriched tissue cultured media with colony stimulating factor which supported the growth of granulopoietic colonies in softagar culture. Mice given 59iron and 99technetium sulfur colloid i.v. showed substantial uptake of both isotopes by the CEM but the 59iron uptake could not be suppressed by hypertransfusion. Surface hematopoietic colony formation on CEM was studied 1-14 days after implantation. A peak colony number occurred at 5 days and the fell off slightly by 2 weeks. These studies indicate that the hematopoietic microenvironment of peritoneally implanted CEM contains a major sub-population of cells with monocytemacrophage features. The hematopoietic microenvironment was well-maintained even though the percentage of monocyte-macrophage marked cells decreased indicating that the microenvironment is not solely dependent upon monocyte-macrophages.
植入小鼠腹腔的纤维素酯膜（CEM）很快就被源自腹膜的细胞覆盖。植入后3 - 5天的峰值点时，高达56%的细胞显示出补体和嗜细胞免疫球蛋白的细胞膜受体。来自CEM的类似比例的细胞在体外吞噬酵母颗粒。在原位研究时，覆盖CEM的细胞中有23%形成了与C3b和IgG包被红细胞的玫瑰花结。在5至17天之间检测到具有单核细胞 - 巨噬细胞特征的细胞百分比逐渐下降。腹膜植入后2周或6周取出的CEM使组织培养基富含集落刺激因子，该因子支持软琼脂培养中粒细胞集落的生长。静脉注射59铁和99锝硫胶体的小鼠显示CEM对这两种同位素都有大量摄取，但高输血不能抑制59铁的摄取。在植入后1 - 14天研究了CEM表面的造血集落形成。集落数量在5天出现峰值，到2周时略有下降。这些研究表明，腹膜植入的CEM的造血微环境包含具有单核细胞 - 巨噬细胞特征的主要细胞亚群。即使标记有单核细胞 - 巨噬细胞的细胞百分比下降，造血微环境仍得到良好维持，这表明微环境并不完全依赖于单核细胞 - 巨噬细胞。
