Colitti Monica, Boschi Federico, Montanari Tommaso
University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences.
Eur J Histochem. 2018 Nov 28;62(4):2984. doi: 10.4081/ejh.2018.2984.
Research on adipobiology has recognized the browning process of white adipocytes as a potential therapeutic strategy for the treatment of obesity and related morbidities. Physical exercise stimulates the secretion of myokines, such as b-aminoisobutyric acid (BAIBA), which in turn promotes adaptive thermogenesis. White adipocyte conversion to brown cells involves dynamic changes in lipid droplet (LD) dimension and in the transcription of brown-specific marker genes. This study analyzes the effect of different doses of BAIBA and at different days of development on 3T3-L1 cells by evaluating morphological changes in LDs and the expression of browning gene markers. Results suggested that the highest concentration of BAIBA after 4 days of differentiation produced the most significant effects. The number of LDs per cell increased in comparison to control cells, whereas the surface area significantly decreased. Brown adipocyte markers were up-regulated, but the effect of treatment was lost at 10 days of differentiation. The thermogenic program induced by BAIBA may reflect a rapid adaptation of adipose tissue to physical exercise. This connection stresses the beneficial impact of physical exercise on metabolic health. The thermogenic program induced by BAIBA may reflect a rapid adaptation of adipose tissue to physical exercise. This connection stresses the beneficial impact of physical exercise on metabolic health.
脂肪生物学研究已认识到白色脂肪细胞的褐色化过程是治疗肥胖症及相关疾病的一种潜在治疗策略。体育锻炼会刺激肌动蛋白的分泌，如β-氨基异丁酸（BAIBA），进而促进适应性产热。白色脂肪细胞向褐色细胞的转化涉及脂滴（LD）大小以及褐色特异性标记基因转录的动态变化。本研究通过评估脂滴的形态变化和褐色化基因标记物的表达，分析了不同剂量的BAIBA以及在不同发育天数对3T3-L1细胞的影响。结果表明，分化4天后最高浓度的BAIBA产生的影响最为显著。与对照细胞相比，每个细胞中脂滴的数量增加，而表面积显著减小。褐色脂肪细胞标记物上调，但在分化10天时治疗效果消失。BAIBA诱导的产热程序可能反映了脂肪组织对体育锻炼的快速适应。这种联系强调了体育锻炼对代谢健康的有益影响。BAIBA诱导的产热程序可能反映了脂肪组织对体育锻炼的快速适应。这种联系强调了体育锻炼对代谢健康的有益影响。
