Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao, 266003, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
BMC Genomics. 2018 Nov 27;19(1):842. doi: 10.1186/s12864-018-5229-1.
Pyropia yezoensis is an important marine crop which, due to its high protein content, is widely used as a seafood in China. Unfortunately, red rot disease, caused by Pythium porphyrae, seriously damages P. yezoensis farms every year in China, Japan, and Korea. Proteomic methods are often used to study the interactions between hosts and pathogens. Therefore, an iTRAQ-based proteomic analysis was used to identify pathogen-responsive proteins following the artificial infection of P. yezoensis with P. porphyrae spores.
A total of 762 differentially expressed proteins were identified, of which 378 were up-regulated and 384 were down-regulated following infection. A large amount of these proteins were involved in disease stress, carbohydrate metabolism, cell signaling, chaperone activity, photosynthesis, and energy metabolism, as annotated in the KEGG database. Overall, the data showed that P. yezoensis resists infection by inhibiting photosynthesis, and energy and carbohydrate metabolism pathways, as supported by changes in the expression levels of related proteins. The expression data are available via ProteomeXchange with the identifier PXD009363.
The current data provide an overall summary of the red algae responses to pathogen infection. This study improves our understanding of infection resistance in P. yezoensis, and may help in increasing the breeding of P. porphyrae-infection tolerant macroalgae.
条斑紫菜是一种重要的海洋作物，由于其高蛋白含量，在中国被广泛用作海鲜。不幸的是，每年在中国、日本和韩国，由原丝体属（Pythium porphyrae）引起的红烂病都会严重破坏条斑紫菜养殖场。蛋白质组学方法常用于研究宿主与病原体之间的相互作用。因此，采用基于 iTRAQ 的蛋白质组学分析方法，对条斑紫菜人工感染原丝体属孢子后的病原体应答蛋白进行了鉴定。
共鉴定出 762 个差异表达蛋白，其中感染后有 378 个上调，384 个下调。这些蛋白大量参与疾病应激、碳水化合物代谢、细胞信号转导、伴侣活性、光合作用和能量代谢等过程，KEGG 数据库注释结果显示。总的来说，数据表明条斑紫菜通过抑制光合作用、能量和碳水化合物代谢途径来抵抗感染，这一结论得到相关蛋白表达水平变化的支持。表达数据可通过 ProteomeXchange 以标识符 PXD009363 获取。
本研究提供了红藻对病原体感染响应的全面总结。该研究提高了我们对条斑紫菜抗感染能力的认识，可能有助于增加对原丝体属感染耐受大型藻类的培育。
