Mokpo Regional Office, National Fishery Products Quality Management Service (NFQS), Mokpo, Jeollanam-do 58746, Korea.
Marine Research Institute, Pusan National University, Busan 46241, Korea.
Plant Dis. 2022 Jan;106(1):30-33. doi: 10.1094/PDIS-07-21-1494-SC. Epub 2022 Jan 20.
Red rot disease is one of the best-known algal diseases infecting red algae species. This disease decreases the quality and quantity of aquaculture products in Korea, Japan, and China. Recently we found that (Oomycetes) infects blades of . Therefore, two species ( and ) have been reported as red rot disease pathogens. In this study, we developed a species-specific molecular marker for distinguishing between the two red rot disease pathogens. Using a polymerase chain reaction restriction fragment length polymorphism method based on the mitochondrial cytochrome c oxidase subunit 2 (2) and nuclear ribosomal RNA large subunit regions, we classified these two species without a sequencing step. This new method had high specificity and efficiency for detecting red rot disease pathogens at the species level for both of the cultured and field samples. Therefore, the molecular markers developed in this study are effective for long-term monitoring of the infection and distribution pattern of each species in aquaculture farms. Moreover, molecular monitoring can provide useful information for predicting infection and preventing mass mortality of species by red rot disease.
红腐烂病是一种广为人知的感染红藻物种的藻类疾病。这种疾病降低了韩国、日本和中国水产养殖产品的质量和数量。最近，我们发现（卵菌）感染了叶片。因此，两种（和）已被报道为红腐烂病病原体。在这项研究中，我们开发了一种用于区分两种红腐烂病病原体的种特异性分子标记。使用基于线粒体细胞色素 c 氧化酶亚基 2（2）和核核糖体 RNA 大亚基区域的聚合酶链反应限制片段长度多态性方法，我们在无需测序步骤的情况下对这两个物种进行了分类。这种新方法对培养和野外样本的两种红腐烂病病原体在种水平上具有高特异性和效率。因此，本研究中开发的分子标记可有效监测水产养殖农场中每种物种的感染和分布模式。此外，分子监测可以提供有关感染预测和防止红腐烂病引起的物种大量死亡的有用信息。
