National Engineering Research Center for Gelatin-based TCM, Dong-E E-Jiao Co., Ltd, 78 E-Jiao Street Donge County, 252201, Shandong Province, China.
National Engineering Research Center for Gelatin-based TCM, Dong-E E-Jiao Co., Ltd, 78 E-Jiao Street Donge County, 252201, Shandong Province, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Biochem Biophys Res Commun. 2019 Jan 1;508(1):237-242. doi: 10.1016/j.bbrc.2018.11.130. Epub 2018 Nov 24.
Donkey milk, similar to human milk in compositions, has been suggested as the best potential hypoallergenic replacement diet for babies suffering from cow milk protein allergens and a promising nutraceutical for aged people. In this study, label-free mass spectrometry analysis was conducted to quantitatively identify the whey proteins differentially expressed in high-milk-yield samples compared with low-milk-yield samples. A total of 216 whey proteins were identified, and 19 of them showed significant differences in high-milk-yield samples. Of these proteins, 16 were upregulated and 3 were downregulated. Differentially expressed proteins (DEPs) were subjected to intensive bioinformatic analysis. Results revealed that the majority of DEPs participated in protein processing in endoplasmic reticulum, estrogen signaling pathway, progesterone-mediated oocyte maturation, and PI3K-Akt signaling pathway. Functional protein analysis suggested that proteins functioned in binding, catalytic activity, molecular function regulation, structural molecule activity, and transporter activity. Our study was the first to analyze the whey protein profile of different samples of donkey milk and to identify candidate proteins that could be used to explore the molecular mechanism related to the yield traits of Dezhou donkey milk. This study provided the biomarkers for the selection of high-milk-yielding donkey and obtained valuable information for future studies.
驴乳在成分上与人类母乳相似，被认为是患牛乳蛋白过敏婴儿的最佳潜在低致敏性替代饮食，也是一种有前途的老年营养保健品。在这项研究中，进行了无标记质谱分析，以定量鉴定高乳产量样本与低乳产量样本中差异表达的乳清蛋白。共鉴定出 216 种乳清蛋白，其中 19 种在高乳产量样本中存在显著差异。其中，16 种上调，3 种下调。对差异表达蛋白（DEPs）进行了深入的生物信息学分析。结果表明，大多数 DEPs 参与内质网中的蛋白质加工、雌激素信号通路、孕激素介导的卵母细胞成熟和 PI3K-Akt 信号通路。功能蛋白分析表明，这些蛋白在结合、催化活性、分子功能调节、结构分子活性和转运体活性方面发挥作用。本研究首次分析了不同驴乳样本的乳清蛋白谱，并鉴定出了候选蛋白，这些蛋白可能用于探索与德州驴乳产量性状相关的分子机制。本研究为高产驴的选择提供了生物标志物，并为未来的研究提供了有价值的信息。
