通过高强度聚焦超声从稳定纳米液滴实现多次药物释放以用于潜在的退行性椎间盘疾病治疗
Multiple-Exposure Drug Release from Stable Nanodroplets by High-Intensity Focused Ultrasound for a Potential Degenerative Disc Disease Treatment.
作者信息
Nguyen Khoi, Pan Hsuan-Yeh, Haworth Kevin, Mahoney Eric, Mercado-Shekhar Karla P, Lin Chia-Ying, Zhang Zhe, C Park Yoonjee
机构信息
Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio, USA.
Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA.
出版信息
Ultrasound Med Biol. 2019 Jan;45(1):160-169. doi: 10.1016/j.ultrasmedbio.2018.09.014. Epub 2018 Oct 26.
The combination of simvastatin and CF680 dye encapsulated by stable nanodroplets has been developed as a drug delivery carrier. Simvastatin has previously been found to be a potential degenerative disc disease treatment. Multiple exposures of the nanodroplets to high-intensity focused ultrasound induced release of simvastatin. Each ultrasound exposure yielded a consistent concentration of the drug and dye released. B-mode ultrasound image analysis data and cavitation data clearly indicated the release mechanism is phase transition of the liquid nanodroplets into gas bubbles. The nanodroplets were stably stored in ex vivo rabbit spinal discs for at least 14 days, and the contents responded to ultrasound exposure on demand. Lastly, nucleus pulposus cells harvested from rabbit spine discs and exposed to media with nanodroplets exhibited a decrease in cell viability (85%) relative to the cells only (96.7%) at 24 h, but no difference at 48 h. Thus, the system may be a potential degenerative disc disease treatment.
由稳定纳米液滴包裹的辛伐他汀和CF680染料的组合已被开发为一种药物递送载体。辛伐他汀此前已被发现是一种潜在的退行性椎间盘疾病治疗药物。纳米液滴多次暴露于高强度聚焦超声会诱导辛伐他汀释放。每次超声暴露都会释放出浓度一致的药物和染料。B超图像分析数据和空化数据清楚地表明，释放机制是液体纳米液滴向气泡的相变。纳米液滴在离体兔椎间盘内稳定储存至少14天，其内容物可根据需要对超声暴露做出反应。最后，从兔椎间盘采集的髓核细胞在24小时时，与仅培养的细胞（96.7%）相比，暴露于含有纳米液滴的培养基中的细胞活力有所下降（85%），但在48小时时没有差异。因此，该系统可能是一种潜在的退行性椎间盘疾病治疗方法。
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