Khan Sher Ali, Liu Lei, Lai Ting, Zhang Ruifen, Wei Zhencheng, Xiao Juan, Deng Yuanyuan, Zhang Mingwei
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, 510610 People's Republic of China.
J Food Sci Technol. 2018 Dec;55(12):4782-4791. doi: 10.1007/s13197-018-3411-8. Epub 2018 Sep 21.
In this study, dried longan pulp (DLP) was subjected to fermentation using selected strains of lactic acid bacteria ( subsp. and ). We then studied changes in the free and bound phytochemical profile, antioxidant activity, free amino acid, and organic acid composition. Fermentation exhibited a 17.4% and 5.7% increase in the amount of free and total phenolic contents of DLP. Phenolic composition determined by HPLC revealed significant changes due to fermentation that were primarily in the contents of gallic acid, vanillic acid, 4-methylcatechol and -coumaric acid, resulting in a 37.9% and 25.7% increase in free gallic acid and 4-methylcatechol, respectively. Fermentation was also found to enhance the ferric reducing antioxidant power of both free and total and the oxygen radical absorbance capacity of free phenolic fraction by 18.3%, 11.8%, and 37.4%, respectively. In addition, fermentation was observed to reduce the contents of free amino acids with bitter taste (phenylalanine, tyrosine and leucine), and increase amino acids (taurine, aspartic acid, cysteine, cysteine thiazoline and γ-amino-butyric acid) having antioxidant potential. Therefore, this study provides basis for the production of fermented longan-based functional products with improved antioxidant activity.
在本研究中,使用选定的乳酸菌菌株(亚种和)对干桂圆肉(DLP)进行发酵。然后,我们研究了游离和结合的植物化学物质概况、抗氧化活性、游离氨基酸和有机酸组成的变化。发酵使DLP的游离酚含量和总酚含量分别增加了17.4%和5.7%。通过高效液相色谱法测定的酚类组成显示,由于发酵,主要是没食子酸、香草酸、4-甲基邻苯二酚和对香豆酸的含量发生了显著变化,导致游离没食子酸和4-甲基邻苯二酚的含量分别增加了37.9%和25.7%。还发现发酵分别使游离酚和总酚的铁还原抗氧化能力以及游离酚部分的氧自由基吸收能力提高了18.3%、11.8%和37.4%。此外,观察到发酵降低了具有苦味的游离氨基酸(苯丙氨酸、酪氨酸和亮氨酸)的含量,并增加了具有抗氧化潜力的氨基酸(牛磺酸、天冬氨酸、半胱氨酸、噻唑啉半胱氨酸和γ-氨基丁酸)。因此,本研究为生产具有改善抗氧化活性的发酵龙眼基功能性产品提供了依据。