Motevalli Haghi Afsaneh, Moradi Sepide, Nateghpour Mehdi, Edrissian Gholamhossein
Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2018 Jul-Sep;13(3):473-479.
Although is usually known as benign malaria, some variations of the parasite can result in acute and sever infection. In this study we tried to determine some genetic variations in AMA-1 antigen among the samples were collected form southeastern Iran.
About two ml blood samples were collected into EDTA pre-dosed tubes from 30 -infected patients individually between 2011 and 2013. A Giemsa stained thick and thin blood film was prepared from each of the patients. A PCR-RFLP technique was employed using EcoR-1, Pvu-II and Hind3 restriction enzymes to determine the allelic variations of the antigen.
A 1300bp gene corresponding to AMA-1 was selected for the amplification process. Among the total cases identified in this study 90% showed similar bounds when exposed to the restriction enzymes. Nine isolates (accession numbers: KF435081-KF435083 and JF682785-JF682790) were identified and registered in Gene bank. Identity among isolates was more than 96% in nucleotide level. Dendrogram clarified a close relationship among the clusters in spite of geographical distribution of the parasite.
This study increased our data about prevalence and variation of AMA-1 alleles amongst isolates in southeastern parts of Iran where besides native population bears considerable Afghan and Pakistani immigrants.
虽然间日疟通常被认为是良性疟疾，但该寄生虫的一些变体可导致急性严重感染。在本研究中，我们试图确定从伊朗东南部采集的样本中AMA-1抗原的一些基因变异。
在2011年至2013年期间，从30名感染患者中分别采集约2ml血液样本至预先装有乙二胺四乙酸（EDTA）的试管中。为每位患者制备了吉姆萨染色的厚血膜和薄血膜。采用PCR-RFLP技术，使用EcoR-1、Pvu-II和Hind3限制性内切酶来确定抗原的等位基因变异。
选择一个与AMA-1相对应的1300bp基因进行扩增过程。在本研究中鉴定的所有病例中，90%在暴露于限制性内切酶时显示出相似的条带。九个分离株（登录号：KF435081-KF435083和JF682785-JF682790）被鉴定并在基因库中注册。分离株之间在核苷酸水平上的同一性超过96%。尽管寄生虫存在地理分布，但系统发育树表明各聚类之间存在密切关系。
本研究增加了我们关于伊朗东南部地区间日疟分离株中AMA-1等位基因流行率和变异的数据，该地区除当地人口外，还有大量阿富汗和巴基斯坦移民。
