Dabelsteen E, Clausen H, Holmstrup P, Reibel J
Department of Oral Diagnosis, Royal Dental College, Copenhagen, Denmark.
APMIS. 1988 Sep;96(9):813-9. doi: 10.1111/j.1699-0463.1988.tb00948.x.
The distribution of carbohydrate structures related to the ABO(H) blood group antigen system was studied in biopsies from eight squamous cell carcinomas, and eight erythroplakias with epithelial dysplasia. Twenty oral lesions without histological evidence of malignancy (13 lichen planus lesions and 7 homogeneous leukoplakias) were also examined. The distribution of Lex, Ley, H type 2 chain, and N-acetyllactosamine, all type 2 chain carbohydrate structures, was investigated by immunohistological staining using monoclonal antibodies with selected specificity. The histological pattern of expression of these antigens in the benign lesions was similar to that of normal oral mucosa, i.e. expression of: N-acetyllactosamine on basal cells, H antigen on parabasal cells, and Lex and Ley on spinous cells. However, lesions with epithelial dysplasia showed H antigen on all spinous cells, and often also on basal cells, with expression of Lex and Ley restricted to the most superficial part of the epithelium above the H-positive cell layers. In carcinomas most cells were negative for H antigen but were positive for Ley and Lex in 5 out of 8 cases.
对8例鳞状细胞癌和8例伴有上皮发育异常的红斑进行活检，研究与ABO(H)血型抗原系统相关的碳水化合物结构分布。还检查了20例无组织学恶性证据的口腔病变（13例扁平苔藓病变和7例均质型白斑）。使用具有选定特异性的单克隆抗体通过免疫组织化学染色研究Lex、Ley、H2型链和N-乙酰乳糖胺（均为2型链碳水化合物结构）的分布。这些抗原在良性病变中的组织学表达模式与正常口腔黏膜相似，即：基底细胞表达N-乙酰乳糖胺，副基底细胞表达H抗原，棘细胞表达Lex和Ley。然而，伴有上皮发育异常的病变在所有棘细胞上均显示H抗原，在基底细胞上也常显示，Lex和Ley的表达局限于H阳性细胞层上方上皮的最表层。在癌组织中，大多数细胞H抗原呈阴性，但8例中有5例Ley和Lex呈阳性。
