Dabelsteen E, Vedtofte P, Hakomori S, Young W W
Cancer Res. 1983 Mar;43(3):1451-4.
Epithelial cell membrane-bound blood group antigens A and B are lost in premalignant and malignant oral lesions. We now show that this loss in premalignant lesions is accompanied by accumulation of a blood group antigen precursor. The precursor structures were: type 2 chain H-antigen, Fuc alpha 1 leads to 2 Gal beta 1 leads to 4 GlcNAc-R (A and B precursor); and N-acetyllactosamine, Gal beta 1-4GlcNAc-R (H-precursor), in which Fuc is L-fucose, Gal is galactose, and GIcNAc is N-acetyl-D-glucosamine. They were demonstrated in tissue sections by immunohistochemical staining techniques with monoclonal antibodies to H-antigen and N-acetyllactosamine. Precursors were found only on basal and suprabasal cells of normal mucosa. In all nine of ten premalignant lesions, the H-antigen was found on all cell membranes in the epithelium, in higher titers than in normal adjacent epithelium. Ten carcinomas were studied, and all showed an irregular distribution of H-antigen. N-Acetyllactosamine was not found in premalignant or malignant lesions. The accumulation of type 2 chain H-antigen in oral premalignant lesions may prove helpful in early diagnosis of epithelial cancer.
上皮细胞膜结合的血型抗原A和B在口腔癌前病变和恶性病变中消失。我们现在表明，癌前病变中的这种消失伴随着血型抗原前体的积累。前体结构为：2型链H抗原，Fucα1→2Galβ1→4GlcNAc-R（A和B前体）；以及N-乙酰乳糖胺，Galβ1-4GlcNAc-R（H前体），其中Fuc为L-岩藻糖，Gal为半乳糖，GlcNAc为N-乙酰-D-葡萄糖胺。通过使用针对H抗原和N-乙酰乳糖胺的单克隆抗体的免疫组织化学染色技术在组织切片中证实了它们。前体仅在正常黏膜的基底细胞和基底上层细胞上发现。在十个癌前病变中的九个中，在上皮细胞的所有细胞膜上都发现了H抗原，其滴度高于相邻的正常上皮。研究了十个癌，所有癌均显示H抗原分布不规则。在癌前或恶性病变中未发现N-乙酰乳糖胺。口腔癌前病变中2型链H抗原的积累可能有助于上皮癌的早期诊断。
