Molecular Biology Unit, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.
University of Louisville, 505 South Hancock Street # 323, Clinical Translational Research Bldg, Louisville, KY, 40202, USA.
Probiotics Antimicrob Proteins. 2019 Sep;11(3):910-920. doi: 10.1007/s12602-018-9489-5.
Probiotic lactobacilli have an unprecedented history of safe use, although some cases of infections have raised concerns about their safety, and hence, a rigorous screening of any new strain even of Lactobacillus is a must in order to study possible adverse interactions with the host, particularly under unhealthy conditions. The present study was, therefore, undertaken to investigate the safety as well as therapeutic efficacy of probiotic Lactobacillus plantarum MTCC 5690 and L. fermentum MTCC 5689 strains in dextran sodium sulfate (DSS)-induced colitis mouse model. Both MTCC 5690 and MTCC 5689 did not induce any detrimental effect on the colitic mice, as was reflected by normal colon and caecum length, blood biochemistry, hematology, and absence of inflammation. Although translocation of both the strains was observed in extraintestinal organs, probiotic-fed mice had significantly improved intestinal permeability and decreased myeloperoxidase (MPO) activity. Probiotic interventions also led to an improved health index and better growth of colitis mice compared to colitis animals with no probiotic intervention. These results point towards the safe use of L. plantarum MTCC 5690 and L. fermentum MTCC 5689 as biotherapeutics for amelioration of inflammatory conditions after establishing their efficacy in human clinical trials.
益生菌乳杆菌具有史无前例的安全使用记录，尽管有些感染病例引起了对其安全性的关注，因此，即使是对乳酸杆菌的任何新菌株，也必须进行严格的筛选，以研究其与宿主之间可能存在的不良相互作用，特别是在不健康的条件下。因此，本研究旨在研究益生菌植物乳杆菌 MTCC 5690 和 L. fermentum MTCC 5689 菌株在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型中的安全性和治疗功效。MTCC 5690 和 MTCC 5689 菌株均未对结肠炎小鼠产生任何不利影响，这反映在结肠和盲肠长度正常、血液生物化学、血液学和无炎症方面。尽管两种菌株都在肠外器官中发生了易位，但益生菌喂养的小鼠的肠道通透性明显改善，髓过氧化物酶（MPO）活性降低。与没有益生菌干预的结肠炎动物相比，益生菌干预还导致结肠炎小鼠的健康指数提高和生长状况改善。这些结果表明，L. plantarum MTCC 5690 和 L. fermentum MTCC 5689 可安全用作生物治疗剂，以改善人类临床试验中的炎症状况。
