Department of Pathology, First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, China.
Department of Histology and Embryology, Hebei North University, 11 Zuanshi South Road, Zhangjiakou, 075000, Hebei, China.
Pathol Oncol Res. 2019 Oct;25(4):1645-1652. doi: 10.1007/s12253-018-0558-6. Epub 2018 Nov 27.
Tripartite motif-containing protein 28 (TRIM28) has been proved to accelerate cell proliferation and metastasis in a variety of human cancers. However, the role of TRIM28 in esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, to compare the biological effect and significance of TRIM28 expression in ESCC, immunohistochemistry (streptavidin-perosidase, S-P) method was used firstly to examine the expression of TRIM28 in 136 cases of ESCC, 35 cases of high grade intraepithelial neoplasia (HGIN), 29 cases of low grade intraepithelial neoplasia (LGIN) and 37 cases of normal esophageal epithelium (NEE). Then the associations of TRIM28 expression with clinicopathological data and overall survival (OS) were also analyzed. Western blot was performed to evaluate TRIM28 protein in a total of 20 matched human ESCC and NEE tissues. Moreover, the localization of TRIM28 protein in ESCC and NEE tissues was also detected by immunofluorescence. TRIM28 protein was mainly distributed in the nucleus of ESCC. The expression of TRIM28 increased progressively from NEE to LGIN, to HGIN, and to ESCC, and it was also related to invasive depth, pTNM stage and lymph node metastasis in ESCC (P < 0.05). The results of western blot and immunofluorescence all showed that the relative expression of TRIM28 protein was markedly upregulated in ESCC compared with the NEE tissues (P < 0.01). However, prognostic analysis showed that TRIM28 may not be a prognostic factor of patients with ESCC. In conclusion, the overexpression of TRIM28 may play an important role for development and metastasis in ESCC.
三结构域蛋白 28（TRIM28）已被证明可促进多种人类癌症中的细胞增殖和转移。然而，TRIM28 在食管鳞状细胞癌（ESCC）中的作用尚不清楚。在这项研究中，为了比较 TRIM28 表达在 ESCC 中的生物学效应和意义，首先使用免疫组织化学（链霉亲和素-过氧化物酶，S-P）方法检测了 136 例 ESCC、35 例高级别上皮内瘤变（HGIN）、29 例低级别上皮内瘤变（LGIN）和 37 例正常食管上皮（NEE）中 TRIM28 的表达。然后还分析了 TRIM28 表达与临床病理数据和总生存（OS）的关系。通过 Western blot 评估了总共 20 对匹配的人 ESCC 和 NEE 组织中的 TRIM28 蛋白。此外，还通过免疫荧光检测了 TRIM28 蛋白在 ESCC 和 NEE 组织中的定位。TRIM28 蛋白主要分布在 ESCC 的核内。TRIM28 的表达从 NEE 到 LGIN、到 HGIN、再到 ESCC 逐渐增加，并且与 ESCC 的浸润深度、pTNM 分期和淋巴结转移有关（P < 0.05）。Western blot 和免疫荧光的结果均表明，与 NEE 组织相比，ESCC 组织中 TRIM28 蛋白的相对表达明显上调（P < 0.01）。然而，预后分析表明，TRIM28 可能不是 ESCC 患者的预后因素。总之，TRIM28 的过表达可能在 ESCC 的发展和转移中起重要作用。
