Morton Simon A, Gupta Sayan, Petzold Christopher J, Ralston Corie Y
Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
Protein Pept Lett. 2019;26(1):70-75. doi: 10.2174/0929866526666181128125725.
Synchrotron hydroxyl radical footprinting is a relatively new structural method used to investigate structural features and conformational changes of nucleic acids and proteins in the solution state. It was originally developed at the National Synchrotron Light Source at Brookhaven National Laboratory in the late nineties, and more recently, has been established at the Advanced Light Source at Lawrence Berkeley National Laboratory. The instrumentation for this method is an active area of development, and includes methods to increase dose to the samples while implementing high-throughput sample delivery methods.
Improving instrumentation to irradiate biological samples in real time using a sample droplet generator and inline fluorescence monitoring to rapidly determine dose response curves for samples will significantly increase the range of biological problems that can be investigated using synchrotron hydroxyl radical footprinting.
同步加速器羟基自由基足迹法是一种相对较新的结构方法，用于研究溶液状态下核酸和蛋白质的结构特征及构象变化。该方法最初于九十年代后期在布鲁克海文国家实验室的国家同步加速器光源处开发，最近在劳伦斯伯克利国家实验室的先进光源处得以确立。此方法的仪器设备是一个活跃的研发领域，包括在实施高通量样品输送方法的同时增加样品剂量的方法。
使用样品液滴发生器实时照射生物样品并通过在线荧光监测快速确定样品的剂量响应曲线来改进仪器设备，将显著扩大可利用同步加速器羟基自由基足迹法研究的生物学问题范围。
