Department of Chemistry, Washington University in St. Louis, St. Louis, Missouri, USA.
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, USA.
Proteomics. 2022 Apr;22(8):e2100222. doi: 10.1002/pmic.202100222.
Structural biology is entering an exciting time where many new high-resolution structures of large complexes and membrane proteins (MPs) are determined regularly. These advances have been driven by over 15 years of technological improvements, first in macromolecular crystallography, and recently in cryo-electron microscopy. Obtaining information about MP higher order structure and interactions is also a frontier, important but challenging owing to their unique properties and the need to choose suitable detergents/lipids for their study. The development of mass spectrometry (MS), both instruments and methodology in the past 10 years, has also advanced it as a complementary method to study MP structure and interactions. In this review, we discuss advances in MS-based footprinting for MPs and highlight recent methodologies that offer new promise for MP study by chemical footprinting and mass spectrometry.
结构生物学正进入一个激动人心的时期，许多新的大型复合物和膜蛋白 (MP) 的高分辨率结构经常被确定。这些进展得益于超过 15 年的技术改进，首先是在大分子晶体学方面，最近在低温电子显微镜方面。获得关于 MP 高级结构和相互作用的信息也是一个前沿领域，由于它们的独特性质以及需要选择合适的去污剂/脂质来研究它们，因此这一领域既重要又具有挑战性。在过去 10 年中，质谱 (MS) 的发展，包括仪器和方法学方面的发展，也使它成为研究 MP 结构和相互作用的一种补充方法。在这篇综述中，我们讨论了基于 MS 的 MP 足迹分析的进展，并强调了最近的方法学，这些方法学为通过化学足迹分析和质谱研究 MP 提供了新的前景。
