Migliaccio A R, Migliaccio G, Adamson J W
Department of Medicine, University of Washington, Seattle 98195.
Blood. 1988 Oct;72(4):1387-92.
We investigated the effects of recombinant interleukin-3 (IL-3), granulocyte-macrophage and granulocyte colony-stimulating factors (GM-CSF and G-CSF), and erythropoietin (Ep) on the number of human hematopoietic progenitors after two to ten days of incubation in liquid cultures deprived of fetal bovine serum (FBS). The source of progenitor cells was normal human marrow depleted of T lymphocytes and/or adherent cells. When adherent cell-depleted marrow was cultured without growth factors, the number of progenitor cells was relatively constant for periods up to eight days. In contrast, a progressive decline in the number of progenitor cells was detected in cultures of nonadherent, T-cell-depleted marrow cells. In both cases, the addition of IL-3 increased by two- to fourfold over input the number of erythroid burst-forming cells (BFU-E) per culture. The number of BFU-E peaked either at day 4 or 8. G-CSF had no effect on the number of progenitor cells per culture. GM-CSF and Ep had no effect in cultures of nonadherent marrow cells but maintained the number of BFU-E in cultures of nonadherent, T-cell-depleted marrow cells. The addition of a neutralizing anti-GM-CSF monoclonal antibody, but not anti-IL-3 neutralizing antiserum, decreased the number of BFU-E in cultures of nonadherent marrow cells. None of the growth factors investigated enhanced the number of GM progenitors to the same degree as the number of BFU-E. However, in cultures of nonadherent, T-cell-depleted marrow cells, IL-3 and GM-CSF maintained the number of GM progenitors up to eight days. These results indicate that IL-3 alone is capable of increasing the number of BFU-E and of maintaining the number of GM progenitors in liquid culture, whereas GM-CSF and Ep are capable of maintaining, but not increasing, BFU-E in this system.
我们研究了重组白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子和粒细胞集落刺激因子（GM-CSF和G-CSF）以及促红细胞生成素（Ep）对在不含胎牛血清（FBS）的液体培养中孵育两到十天后人造血祖细胞数量的影响。祖细胞的来源是去除T淋巴细胞和/或贴壁细胞的正常人骨髓。当去除贴壁细胞的骨髓在无生长因子的情况下培养时，祖细胞数量在长达八天的时间内相对恒定。相比之下，在非贴壁、去除T细胞的骨髓细胞培养物中检测到祖细胞数量逐渐下降。在这两种情况下，添加IL-3使每个培养物中红系爆式集落形成细胞（BFU-E）的数量比接种量增加了两到四倍。BFU-E的数量在第4天或第8天达到峰值。G-CSF对每个培养物中祖细胞的数量没有影响。GM-CSF和Ep在非贴壁骨髓细胞培养物中没有作用，但在非贴壁、去除T细胞的骨髓细胞培养物中维持了BFU-E的数量。添加中和抗GM-CSF单克隆抗体，但不添加抗IL-3中和抗血清，会降低非贴壁骨髓细胞培养物中BFU-E的数量。所研究的生长因子均未像BFU-E数量那样同等程度地增加GM祖细胞的数量。然而，在非贴壁、去除T细胞的骨髓细胞培养物中，IL-3和GM-CSF在长达八天的时间内维持了GM祖细胞的数量。这些结果表明，单独的IL-3能够增加液体培养中BFU-E的数量并维持GM祖细胞的数量，而GM-CSF和Ep能够在该系统中维持但不能增加BFU-E的数量。
