Hendry J H
Int J Radiat Biol Relat Stud Phys Chem Med. 1978 Jan;33(1):47-55. doi: 10.1080/09553007714551481.
Studies on the radionecrosis of mouse tails demonstrate the following modifications to the dose necessary for necrosis in 50 per cent of tails (the ND50): (a) There is very little reduction in ND50 values for irradiated lengths of tail from 2 cm to almost the whole tail, but there is a sharp increase in dose for lengths less than 1.5 cm. (b) The ND50 is high for unanesthetized mice irradiated in air, due to tissue hypoxia. (c) The hypoxia can be reduced by varying amounts by applying heat to the tail, or by flowing oxygen over the tail surface, or by anesthetizing the animal. (d) The ingress of oxygen through the surface can be reduced by placing a clamp round the proximal tail. These features are discussed with reference to the state and possible position of the target cells, and to the use of this assay technique in comparative studies.
对小鼠尾巴放射性坏死的研究表明，对于50%尾巴出现坏死所需的剂量（ND50）有以下变化：(a) 从2厘米到几乎整个尾巴长度进行照射时，ND50值降低很少，但长度小于1.5厘米时剂量会急剧增加。(b) 由于组织缺氧，在空气中照射的未麻醉小鼠的ND50较高。(c) 通过对尾巴加热、在尾巴表面通氧气或麻醉动物，可不同程度地减少缺氧情况。(d) 通过在尾巴近端放置夹子可减少氧气从表面进入。结合靶细胞的状态和可能位置以及该检测技术在比较研究中的应用对这些特征进行了讨论。
