Qi Yingjiao, Wang Zhixing, Wu Fan, Yin Bin, Jiang Tao, Qiang Boqin, Yuan Jiangang, Han Wei, Peng Xiaozhong
State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Medical Primate Research Center, Neuroscience Center, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
J Cell Biochem. 2019 May;120(5):8343-8351. doi: 10.1002/jcb.28117. Epub 2018 Nov 28.
Now, numerous exciting findings have been found that long noncoding RNAs (lncRNAs) play a vital role in cancer malignant progression. However, their potential involvement in glioma is not well understood. Here, we performed a high-throughput microarray to detect the lncRNA expression profiles between glioma cell lines and normal astrocyte cell lines. HOXD-AS2 was increased in glioma cells and it was associated with glioma grade and poor prognosis. Loss of HOXD-AS2 can inhibit glioma cell growth by inducing cell-cycle G1 arrest in vitro. The proliferation of glioma was inhibited followed by knocking down the expression of HOXD-AS2 not only in subcutaneous injection model but also in orthotopic implantation model. These findings indicate that HOXD-AS2 promotes the glioma progression and may serve as a potential target for glioma diagnosis and therapy.
目前，已发现许多令人兴奋的研究结果表明，长链非编码RNA（lncRNAs）在癌症恶性进展中起着至关重要的作用。然而，它们在胶质瘤中的潜在作用尚未得到充分了解。在此，我们进行了一项高通量微阵列实验，以检测胶质瘤细胞系和正常星形胶质细胞系之间的lncRNA表达谱。HOXD-AS2在胶质瘤细胞中表达增加，且与胶质瘤分级和预后不良相关。在体外，HOXD-AS2缺失可通过诱导细胞周期G1期阻滞来抑制胶质瘤细胞生长。敲低HOXD-AS2的表达不仅在皮下注射模型中，而且在原位植入模型中均能抑制胶质瘤的增殖。这些发现表明，HOXD-AS2促进胶质瘤进展，可能成为胶质瘤诊断和治疗的潜在靶点。
