Sun Xunsha, Jin Yulin, Liang Qihua, Tang Jie, Chen Jinsong, Yu Qiuxia, Li Fatao, Li Yan, Wu Jieying, Wu Shaoqing
Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Guangzhou Women and Children's Medical Center, Sun Yat-sen University, Guangzhou, China.
J Clin Lab Anal. 2019 Mar;33(3):e22825. doi: 10.1002/jcla.22825. Epub 2018 Nov 28.
Hypoxic preconditioning alters the biological properties of mesenchymal stem cells (MSCs). It is not known whether this process has an effect on circular RNAs (circRNAs) in MSCs.
Human placental chorionic plate-derived MSCs (hpcpMSCs) isolated from the same placentae were classed into two groups: hypoxic pretreated (hypoxia) group and normally cultured (normoxia) group. The comparative circRNA microarray analysis was used to determine circRNAs expression and verified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in the two groups.
One hundred and two differentially expressed circRNAs in the hypoxia group were found compared to that in the normoxia group (fold change >1.5-fold and P < 0.05). The expression levels of circRNAs by qRT-PCR were consistent with those evaluated by microarray analysis. Gene ontology (GO) analysis showed that the putative function of their target genes for those differentially expressed circRNAs was primarily involved in cell development and its differentiation and regulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that transcriptional misregulation in cancer and mitogen-activated protein kinase (MAPK) signaling pathway were the most significant. MAPK signaling pathway was found to be the core regulatory pathway triggered by hypoxia.
The results indicate that the altered expression of specific circRNAs in MSCs is associated with hypoxic preconditioning. This finding provides further exploration of underlying mechanisms of the characteristic changes of MSCs with hypoxic preconditioning.
缺氧预处理可改变间充质干细胞（MSC）的生物学特性。目前尚不清楚该过程是否会对MSC中的环状RNA（circRNA）产生影响。
从同一胎盘分离的人胎盘绒毛板来源的MSC（hpcpMSC）分为两组：缺氧预处理（缺氧）组和正常培养（常氧）组。采用比较性circRNA微阵列分析确定两组中circRNA的表达情况，并通过定量逆转录聚合酶链反应（qRT-PCR）进行验证。
与常氧组相比，缺氧组发现102种差异表达的circRNA（倍数变化>1.5倍且P<0.05）。qRT-PCR检测的circRNA表达水平与微阵列分析评估的结果一致。基因本体（GO）分析表明，这些差异表达circRNA的靶基因的推定功能主要涉及细胞发育及其分化和调控。京都基因与基因组百科全书（KEGG）通路分析显示，癌症中的转录失调和丝裂原活化蛋白激酶（MAPK）信号通路最为显著。发现MAPK信号通路是缺氧触发的核心调控通路。
结果表明，MSC中特定circRNA的表达改变与缺氧预处理有关。这一发现为进一步探索缺氧预处理后MSC特征性变化的潜在机制提供了依据。
