1Institute of Dermatology, Taiyuan City Centre Hospital, Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi Province 030009, China.
Eur J Dermatol. 2019 Feb 1;29(1):29-38. doi: 10.1684/ejd.2018.3483.
Circular RNAs (circRNAs) have recently emerged as novel non-coding regulatory RNAs, reportedly involved in many biological processes and human diseases. However, the role of circRNAs in the pathogenesis of psoriasis is unclear. Additionally, mesenchymal stem cells (MSCs) are involved in pathological processes associated with immune diseases, including psoriasis. In this study, we determined the circRNA expression profile of MSCs from psoriatic skin lesions and investigated possible mechanisms associated with psoriasis. RNA sequencing was used to detect circRNA expression in MSCs from psoriatic skin lesions and normal skin, and detailed bioinformatic analysis was performed. Our results allowed us to predict a circRNA-microRNA (miRNA) interaction network and subsequently validate seven differentially expressed circRNAs and three miRNAs by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, we analysed circRNA expression in plasma to determine the extent of abnormal circRNA circulation in patients with psoriasis. In total, 6,323 circRNAs were detected, of which 3,227 constituted previously unreported circRNAs. We identified 129 circRNAs that exhibited significantly different expression patterns between the psoriasis and control groups, and established a circRNA-miRNA interaction network through bioinformatic analysis that indicated circRNA interaction with miRNAs associated with psoriasis. Additionally, qRT-PCR results confirmed the differential expression of seven circRNAs as well as the stable expression of three circRNAs in plasma from psoriasis patients, consistent with trends observed in cells. This is the first study of circRNA expression in psoriasis, indicating possible circRNA involvement in the pathogenesis of psoriasis.
环状 RNA（circRNAs）最近被认为是一种新型的非编码调节 RNA，参与许多生物过程和人类疾病。然而，circRNAs 在银屑病发病机制中的作用尚不清楚。此外，间充质干细胞（MSCs）参与与免疫疾病相关的病理过程，包括银屑病。在这项研究中，我们确定了银屑病皮损中 MSCs 的 circRNA 表达谱，并研究了与银屑病相关的可能机制。我们使用 RNA 测序来检测银屑病皮损和正常皮肤中 MSCs 的 circRNA 表达，并进行了详细的生物信息学分析。我们的结果允许我们预测 circRNA-miRNA（miRNA）相互作用网络，然后通过定量实时逆转录聚合酶链反应（qRT-PCR）验证 7 个差异表达的 circRNAs 和 3 个 miRNAs。此外，我们分析了血浆中的 circRNA 表达，以确定银屑病患者中异常 circRNA 循环的程度。总共检测到 6323 个 circRNAs，其中 3227 个构成了以前未报道的 circRNAs。我们鉴定了 129 个在银屑病组和对照组之间表现出明显不同表达模式的 circRNAs，并通过生物信息学分析建立了 circRNA-miRNA 相互作用网络，表明 circRNA 与与银屑病相关的 miRNAs 相互作用。此外，qRT-PCR 结果证实了 7 个 circRNAs 的差异表达以及 3 个 circRNAs 在银屑病患者血浆中的稳定表达，与细胞中观察到的趋势一致。这是银屑病 circRNA 表达的首次研究，表明 circRNA 可能参与银屑病的发病机制。
