AO Research Institute Davos, 7270 Davos Platz, Switzerland.
Department of Cranio-Maxiofacial Surgery, Medical Center-Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, 79085 Freiburg, Germany.
Cells. 2020 Feb 9;9(2):398. doi: 10.3390/cells9020398.
The goal of the present study is to identify the differential expression of circular RNA (circRNA), miRNA, and piwi-interacting RNA (piRNA) after lineage commitment towards osteo- and chondrogenesis of human bone marrow mesenchymal stromal cells (hMSCs). The cells were maintained for 7 days in either osteogenic or chondrogenic medium. RNA sequencing was performed to assess the expression of miRNA and piRNA, while RNA hybridization arrays were used to identify which circRNA were differentially expressed. qPCR validation of a selection of targets for both osteogenic and chondrogenic differentiation was carried out. The differential expression of several circRNA, miRNA, and piRNA was identified and validated. The expression of total and circular isoforms of was upregulated both in osteo- and chondrogenesis and it was influenced by the presence of dexamethasone. , and were also identified as regulated in differentiation and/or by dexamethasone. In conclusion, we have identified a set of different non-coding RNAs that are differentially regulated in early osteogenic and chondrogenic differentiation, paving the way for further investigation to understand how dexamethasone controls the expression of those genes and what their function is in MSC differentiation.
本研究的目的是鉴定人骨髓间充质基质细胞（hMSCs）向成骨和成软骨分化后，环状 RNA（circRNA）、miRNA 和 piRNA 的差异表达。将细胞在成骨或成软骨培养基中维持 7 天。进行 RNA 测序以评估 miRNA 和 piRNA 的表达，而 RNA 杂交阵列则用于鉴定差异表达的 circRNA。对成骨和成软骨分化的一些靶基因进行了 qPCR 验证。鉴定并验证了几种 circRNA、miRNA 和 piRNA 的差异表达。在成骨和成软骨分化中， 和 的总表达和环状异构体均上调，并且受地塞米松的影响。 、 和 也被鉴定为在分化过程中受调节，或受地塞米松调节。总之，我们已经鉴定出一组在早期成骨和成软骨分化中差异调节的不同非编码 RNA，为进一步研究地塞米松如何控制这些基因的表达及其在 MSC 分化中的功能铺平了道路。
