Greenway C V, Pushka K, Sitar D S, Rogers L K
Department of Pharmacology, University of Manitoba, Winnipeg, Canada.
Can J Physiol Pharmacol. 1988 Apr;66(4):307-11. doi: 10.1139/y88-052.
The alcohol oxidase membrane technique is available for measurement of ethanol in commercial fluids. In this paper we examined its usefulness for cat and human blood in comparison with gas-liquid chromatography (GLC). The membrane method proved to be simple, reproducible, accurate, and inexpensive. Analysis took 1-2 min per sample and required only 25 microL of whole blood for measurement of concentrations between 0.05 and 1.0 mM (0.25-5 mg/dL) and 10 microL of whole blood for measurement of concentrations between 1.0 and 40 mM (5-190 mg/dL). Background concentrations were undetectable in cats after extraneous sources of alcohols were removed. The alcohol oxidase membrane technique is less specific than GLC, but it may be useful when ethanol is administered after background samples have shown an absence of other nonspecific reactants. Its high sensitivity is useful for kinetic studies where blood ethanol concentrations are below or close to those required for maximal hepatic ethanol metabolism.
酒精氧化酶膜技术可用于测定商业流体中的乙醇。在本文中，我们将其与气液色谱法（GLC）相比较，检验了该技术对猫和人血液的适用性。结果证明，膜法操作简单、结果可重复、准确且成本低廉。每个样本的分析耗时1 - 2分钟，测定浓度在0.05至1.0 mM（0.25 - 5 mg/dL）之间时仅需25微升全血，测定浓度在1.0至40 mM（5 - 190 mg/dL）之间时仅需10微升全血。去除外来酒精来源后，在猫身上未检测到背景浓度。酒精氧化酶膜技术的特异性不如GLC，但当背景样本显示不存在其他非特异性反应物后再给予乙醇时，该技术可能会有用。其高灵敏度对于血液乙醇浓度低于或接近肝脏乙醇最大代谢所需浓度的动力学研究很有用。
