用于检测细胞附近细胞外分析物的脂质体平台。
A Liposomal Platform for Sensing of Extracellular Analytes Near Cells.
机构信息
ARC Centre of Excellence for Nanoscale BioPhotonics, Institute of Photonics and Advanced Sensing, Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide SA 5005, Australia.
Discipline of Physiology, Faculty of Health Sciences, The University of Adelaide, Adelaide SA 5005, Australia.
出版信息
Biosensors (Basel). 2018 Nov 26;8(4):117. doi: 10.3390/bios8040117.
Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca, GSH, and Zn, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca (0.25 mM⁻1 mM), Zn (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn (1 mM), Ca (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications.
细胞通透性荧光化学传感器（钙黄绿素、单氯荧光素和最近报道的基于螺吡喃的传感器 SP2）已被整合到酵母总脂提取物为基础的脂质体中，以抑制细胞的固有通透性，从而分别检测细胞外的 Ca、GSH 和 Zn。经过重新设计的传感器具有增强的水溶性和在水介质中定量测量生物相关浓度的 Ca（0.25 mM⁻1 mM）、Zn（6.25 µM⁻50 µM）和 GSH（0.25 mM⁻1 mM）的能力，通过荧光实现。此外，脂质体传感器对 HEK293 细胞无毒，并且能够在不内化的情况下检测细胞附近的外源性添加的 Zn（1 mM）、Ca（1 mM）或 GSH（1 mM）。这个新的传感平台提供了一种方法，可以重新设计一系列细胞内荧光传感器来专门检测细胞外分析物，同时提高生物相容性，以在广泛的生物医学应用中得到更广泛的应用。
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