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利用响应面法优化药用真菌抑曲霉素产生菌的贮藏条件。

Optimization of Storage Conditions of the Medicinal Herb against the Sterigmatocystin Producer Using Response Surface Methodology.

机构信息

Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

Ministry of Education Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou 510006, China.

出版信息

Toxins (Basel). 2018 Nov 27;10(12):499. doi: 10.3390/toxins10120499.

DOI:10.3390/toxins10120499
PMID:30486344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6316290/
Abstract

The root of is a commonly used herb in Southern China, and also constitutes the main raw material of Canton herbal tea. is readily contaminated by mildew because of rich nutrients. producing sterigmatocystin is one of the most common molds that contaminate foodstuffs and medicinal herbs. Previous study on the evaluation of fungal contamination on samples of revealed that was the dominant contaminant. In this study, experiments based on response surface methodology combined with central composite design were carried out to determine the optimal storage conditions of to minimize the contamination of sterigmatocystin. The herb, manually innoculated with was stored under different temperatures (20⁻40 °C) and humidity (80⁻95%) for seven days. The effects of temperature and humidity were evaluated using total saponin, polysaccharide and the sterigmatocystin levels as quality indexes. The results showed that grew quickly and produced large amounts of sterigmatocystin on , at humidity ranging from 85% to 90% and temperatures above 26 °C. Meanwhile, total saponin and polysaccharide amounts were reduced significantly. These findings suggested that samples should be stored in an environment with humidity and temperature below 85% and 26 °C, respectively, to reduce growth and sterigmatocystin production.

摘要

是一种常见的南方草药,也是广东凉茶的主要原料。由于营养丰富,很容易受到霉菌的污染。 产生的杂色曲霉素是污染食品和草药最常见的霉菌之一。以前对 的真菌污染评估研究表明, 是主要污染物。在这项研究中,基于响应面法结合中心复合设计的实验被用来确定 的最佳储存条件,以最大限度地减少杂色曲霉素的污染。将手动接种 的草药在不同温度(20⁻40°C)和湿度(80⁻95%)下储存七天。使用总皂苷、多糖和杂色曲霉素水平作为质量指标来评估温度和湿度的影响。结果表明,在湿度为 85%至 90%且温度高于 26°C的 上, 生长迅速,产生大量的杂色曲霉素。同时,总皂苷和多糖的含量明显降低。这些发现表明, 样品应储存在湿度和温度分别低于 85%和 26°C的环境中,以减少 的生长和杂色曲霉素的产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/a24ebbc8f4ab/toxins-10-00499-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/4e91d3dffbd9/toxins-10-00499-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/8c5e4beb03cf/toxins-10-00499-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/30abdfd1161c/toxins-10-00499-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/22cbe4e92096/toxins-10-00499-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/a24ebbc8f4ab/toxins-10-00499-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/4e91d3dffbd9/toxins-10-00499-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/8c5e4beb03cf/toxins-10-00499-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/30abdfd1161c/toxins-10-00499-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/22cbe4e92096/toxins-10-00499-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bcd1/6316290/a24ebbc8f4ab/toxins-10-00499-g005.jpg

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