基于非典型猪瘟病毒 E2 蛋白的亚单位疫苗诱导小鼠产生 Th2 型免疫应答。

A Subunit Vaccine Based on E2 Protein of Atypical Porcine Pestivirus Induces Th2-type Immune Response in Mice.

机构信息

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

出版信息

Viruses. 2018 Nov 28;10(12):673. doi: 10.3390/v10120673.

An atypical porcine pestivirus (APPV) causing congenital tremor type A-II in piglets was identified in China in 2016. An increased number of cases of APPV have been reported in various countries all over the world since 2015. This study aimed to develop an effective subunit vaccine against APPV based on the E2 protein, which is the main immunogenicity protein of APPV. In this study, E2 protein was successfully expressed by the baculovirus expression system. E2 protein was confirmed by Western blot assay, which showed that E2 protein possesses N-linked glycosylation sites. The immunogenicity of E2 subunit vaccine was evaluated in mice. The E2 protein emulsified with ISA 201VG adjuvant induced significantly higher levels of APPV-specific antibodies and elicited stronger lymphocyte proliferative responses and higher interleukin-10 secretion than those of the E2 protein emulsified with IMS 1313VG adjuvant. This observation indicates that the E2 subunit vaccine induces a Th2-type immune response. Our results showed that E2 protein can be developed as a safe and effective subunit vaccine for the control of APPV infection.

一种引起仔猪先天性震颤 A-II 型的非典型猪瘟病毒（APPV）于 2016 年在中国被鉴定。自 2015 年以来，世界各国报告了越来越多的 APPV 病例。本研究旨在基于 APPV 的主要免疫原性蛋白 E2 蛋白，开发针对 APPV 的有效亚单位疫苗。本研究通过杆状病毒表达系统成功表达了 E2 蛋白。Western blot 分析证实了 E2 蛋白具有 N 连接糖基化位点。在小鼠中评估了 E2 亚单位疫苗的免疫原性。与用 IMS 1313VG 佐剂乳化的 E2 蛋白相比，用 ISA 201VG 佐剂乳化的 E2 蛋白诱导了更高水平的 APPV 特异性抗体，并引起了更强的淋巴细胞增殖反应和更高的白细胞介素-10 分泌。这一观察表明，E2 亚单位疫苗诱导了 Th2 型免疫反应。我们的结果表明，E2 蛋白可以开发为控制 APPV 感染的安全有效的亚单位疫苗。