Faculdade de Veterinária, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Faculdade de Veterinária, Setor de Patologia Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Transbound Emerg Dis. 2018 Feb;65(1):22-26. doi: 10.1111/tbed.12753. Epub 2017 Nov 8.
Recently, a putative new pestivirus species, provisionally named as Atypical Porcine Pestivirus (APPV), was associated with the congenital tremor in piglets in North America and consequently in Europe and Asia. The present research aimed to describe the detection and characterization of APPV employing NS5B gene partial sequencing, gross pathology and histologic examination of piglets displaying congenital tremor from two different farms of Southern Brazil. No gross lesions were observed, and the histological findings revealed moderate vacuolization of the white matter of the cerebellum. RT-PCR followed by DNA sequencing and a phylogenetic analysis confirmed the presence of APPV in samples from the two farms, which the samples were distinct in nature. Phylogenetic reconstruction reinforced the high genetic variability within the APPVs previously reported. This is the first report of APPV in South America suggesting that this new group of viruses may be widespread in swine herds in other countries as it is in Brazil.
最近，一种假定的新型瘟病毒种，暂时命名为非典型猪瘟病毒（APPV），与北美的仔猪先天性震颤有关，随后在欧洲和亚洲也有报道。本研究旨在描述 APPV 的检测和特征，采用 NS5B 基因部分测序、来自巴西南部两个不同农场显示先天性震颤的仔猪的大体病理和组织学检查。未观察到明显的大体病变，组织学发现小脑白质有中度空泡化。RT-PCR 后进行 DNA 测序和系统发育分析证实了两个农场样本中 APPV 的存在，这些样本在性质上是不同的。系统发育重建加强了先前报道的 APPV 内的高遗传变异性。这是 APPV 在南美洲的首次报告，表明该新病毒群可能像在巴西一样广泛存在于其他国家的猪群中。
