São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Preventive Veterinary Medicine and Animal Reproduction, Jaboticabal, SP, Brazil.
Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Transbound Emerg Dis. 2018 Apr;65(2):375-380. doi: 10.1111/tbed.12824. Epub 2018 Feb 2.
Atypical porcine pestivirus (APPV) has been detected in piglets with congenital tremor (CT) from three different continents including North America, Europe and Asia. Thirteen piglets from four farms in two different states in Brazil with CT were sampled. Viral RNA was detected by quantitative real-time PCR in the cerebellum or cerebellum and spinal cord in the 100% of the piglets with CT, and APPV was not detected in any tissue sample from clinically non-affected piglets with the exception of the cerebellum of one piglet from Farm A. Piglets with CT had an odds ratio of 99.0 (95% CI 3.4, 2823.8; p = .0072) compared to piglets without CT to test positive for APPV by qRT-PCR. A subset of positive samples was selected for sequencing of the NS3 gene. Phylogenetic analysis revealed that Brazilian sequences of the NS3 formed an independent cluster and had the highest sequence identity with a sequence from the United States. This is the first identification of APPV infection in piglets with CT in South America.
已从包括北美洲、欧洲和亚洲在内的三个不同大洲的先天性震颤（CT）仔猪中检测到非典型猪瘟病毒（APPV）。从巴西两个不同州的四个农场的 13 头具有 CT 的仔猪中采集了样本。100%的 CT 仔猪的小脑或小脑和脊髓中通过定量实时 PCR 检测到病毒 RNA，除了来自农场 A 的一头仔猪的小脑外，未在任何临床无明显症状的仔猪的组织样本中检测到 APPV。与无 CT 的仔猪相比，具有 CT 的仔猪通过 qRT-PCR 检测到 APPV 呈阳性的可能性比为 99.0（95%CI 3.4，2823.8；p=0.0072）。选择了一组阳性样本进行 NS3 基因测序。系统进化分析表明，巴西 NS3 序列形成了一个独立的聚类，与来自美国的序列具有最高的序列同一性。这是首次在南美洲发现具有 CT 的仔猪感染 APPV。
