Lin Y L, Chen S T, Gan L J, Jiang L, Zhang W Q, Zen Y B, Wang Z, Gao L Q, Liang X H, Ou Q S, Yang B
Department of Clinical Laboratory of the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China.
Zhonghua Yi Xue Za Zhi. 2018 Nov 27;98(44):3607-3612. doi: 10.3760/cma.j.issn.0376-2491.2018.44.012.
To evaluate the performance of MALDI Biotyper system in identification of clinically isolated pathogens so as to provide a new rapid identification method. Total 21 270 pathogens strains, isolated from the First Affiliated Hospital of Fujian Medical Universityduring Nov. 2015 to Dec. 2016, were identified by VITEK-Ⅱ, API and MALDI Biotyper system, respectively.The isolated strains were confirmed by DNA sequencing. The identification of common bacteria with MALDI Biotyper and phenotypic system is highly consistent (>95% and >90%). Among 43 strains of anaerobic bacteria, MALDI Biotyper could identify 90.7% bacteria to species level and 97.7% bacteria to genus level with the statistical significance(χ(2)=6.76, <0.01), while phenotypic system only identified 65.1% bacteria to species and 69.8% bacteria to genus. Also, no statistical significance was shown for and (>0.05). MALDI Biotyper could identify 76% and all of and to genus level. MALDI Biotyper is an easy-performed, sensitive method for the identification of clinically isolated pathogens. Additionally, the pretreatment and reference database has the effect on identification.
