a Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences , Nankai University , Tianjin , China.
J Biomater Sci Polym Ed. 2019 Apr;30(5):337-354. doi: 10.1080/09205063.2018.1553104. Epub 2019 Feb 21.
Adsorbents are widely used in hemoperfusion for bilirubin removal. However, their performance is often compromised by the presence of plasma proteins. In this study, the bilirubin adsorption capacity of polyvinyl alcohol microspheres (PVAm) functionalized with different amino-alkane ligands has been investigated, with the aim of gaining binding selectivity over albumin. Octylamine-functionalized PVA microspheres (PVAm-8) exhibited an excellent adsorption capacity for bilirubin (75% and 3.95 mg/mL in PBS vs 72% and 3.84 mg/mL in albumin solution) when compared to the clinical adsorbent BPR (92% and 4.84 mg/mL in PBS vs 71%, and 3.80 mg/mL in albumin solution). The bilirubin adsorption capacities of PVAm-8 were largely unaffected by the presence of albumin. Adsorption of bilirubin to PVAm-8 occurs mainly through hydrophobic effects, with adsorption consistent with the monolayer model and the pseudo-first-order model operating in both PBS and albumin solution. The effects of PVAm-8 on hemolytic activity, blood component stability and coagulant activity were negligible, indicating that PVAm-8 has good potential as a high-affinity bilirubin adsorbent for hemoperfusion applications.
吸附剂在胆红素的血液灌流中被广泛应用。然而，其性能往往受到血浆蛋白的影响。本研究考察了不同的氨基烷烃配体功能化的聚乙烯醇微球（PVAm）对胆红素的吸附性能，旨在获得对白蛋白的结合选择性。与临床吸附剂 BPR 相比，辛胺功能化的 PVA 微球（PVAm-8）在 PBS 中对胆红素的吸附容量（75%和 3.95mg/mL，与 72%和 3.84mg/mL 在白蛋白溶液中）表现出优异的吸附能力。与白蛋白相比，PVAm-8 对胆红素的吸附容量影响不大。PVAm-8 对胆红素的吸附主要通过疏水作用进行，吸附符合 PBS 和白蛋白溶液中的单层模型和准一级模型。PVAm-8 对溶血活性、血液成分稳定性和凝血活性的影响可以忽略不计，这表明 PVAm-8 作为血液灌流用高亲和力胆红素吸附剂具有良好的应用潜力。
