Ma Chun Fang, Gao Qiang, Xia Kai Sheng, Huang Zhi Yuan, Han Bo, Zhou Cheng Gang
Department of Chemistry, Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan, 430074, PR China.
Department of Chemistry, Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan, 430074, PR China.
Colloids Surf B Biointerfaces. 2017 Jan 1;149:146-153. doi: 10.1016/j.colsurfb.2016.10.015. Epub 2016 Oct 11.
The development of bilirubin adsorbents with high adsorption efficiencies towards albumin-bonded bilirubin is still a considerable challenge. In this work, a three-dimensionally porous graphene (3D-pGR) has been fabricated through a simple carbon dioxide (CO) activation of thermally exfoliated graphite oxide (EGO). Intriguingly, the resultant 3D-pGR material showed hierarchically micro-meso-macroporous structure, high specific surface area of up to 843mg, and large pore volume as high as 2.71cmg. Besides, the large planar π-configuration structure of 3D-pGR made it possible to compete effectively with albumin for bilirubin binding. Taking advantages of these fantastic characteristics, the 3D-pGR was demonstrated to be extraordinarily efficient for bilirubin removal from a bovine serum albumin (BSA)-rich solution. Under optimized conditions, the maximum adsorption capacity of 3D-pGR for BSA-bonded bilirubin was up to 126.1mgg, which is not only significantly higher than the adsorption capacities of currently available adsorbents towards albumin-bonded bilirubin, but also superior to those of many reported adsorbents towards free bilirubin. In addition, the hemolysis assay of 3D-pGR indicated that this material had negligible hemolysis effect. Findings from this study may open up important new possibilities for removal of protein-bonded toxins.
开发对与白蛋白结合的胆红素具有高吸附效率的胆红素吸附剂仍然是一项重大挑战。在这项工作中，通过对热剥离氧化石墨（EGO）进行简单的二氧化碳（CO）活化制备了三维多孔石墨烯（3D-pGR）。有趣的是，所得的3D-pGR材料呈现出分级的微-介-大孔结构，比表面积高达843mg，孔体积大至2.71cmg。此外，3D-pGR的大平面π构型结构使其能够与白蛋白有效竞争胆红素结合。利用这些出色的特性，3D-pGR被证明对从富含牛血清白蛋白（BSA）的溶液中去除胆红素非常有效。在优化条件下，3D-pGR对与BSA结合的胆红素的最大吸附容量高达126.1mgg，这不仅显著高于目前可用吸附剂对与白蛋白结合的胆红素的吸附容量，而且优于许多报道的吸附剂对游离胆红素的吸附容量。此外，3D-pGR的溶血试验表明该材料的溶血作用可忽略不计。这项研究的结果可能为去除与蛋白质结合的毒素开辟重要的新可能性。
