Instituto de Magnetismo Aplicado, 'Salvador Velayos', UCM-CSIC-ADIF, Las Rozas, P.O. Box 155, Madrid 28230, Spain.
Laboratorio de Microscopias Avanzadas (LMA), Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Mariano Esquillor s/n, 50018 Zaragoza, Spain.
J Nanosci Nanotechnol. 2019 Apr 1;19(4):2008-2015. doi: 10.1166/jnn.2019.15313.
In this study, we evaluate the magnetic properties and cytotoxic effect of magnetic nanoparticles (MNPs) based on magnetite and Mn, Co and Ni ferrites, obtained by electrochemical synthesis. These nanoparticles have almost spherical shape and an mode size of 9±1 nm. The electrochemical synthesis produces a single crystallographic phase with a spinel-like structure in all cases. Magnetization saturation at room temperature varies with the composition of the ferrites from (Fe₃O₄) > (MnFe₂O₄) > (CoFe₂O₄) > (NiFe₂O₄). Ferrite MNPs present low magnetic remanence indicating a superparamagnetic-like response at room temperature. However, the different values of magnetic anisotropy and size produce variations in the values of coercivity and susceptibility of the ferrite MNPs. The cytotoxicity of the different ferrites was evaluated by internalizing MNP in HeLa cancer cells. Although magnetite and Mn ferrite present low toxicity for all the concentrations studied, significant cytotoxic effect were observed when incubating the cells with high concentration of Co and Ni ferrites.
在这项研究中，我们评估了基于磁铁矿和 Mn、Co 和 Ni 铁氧体的通过电化学合成得到的磁性纳米粒子（MNPs）的磁性和细胞毒性效应。这些纳米粒子具有几乎球形的形状和 9±1nm 的模态尺寸。电化学合成在所有情况下都产生具有尖晶石状结构的单相结晶相。室温下的磁化饱和随铁氧体的组成而变化，从 (Fe₃O₄) > (MnFe₂O₄) > (CoFe₂O₄) > (NiFe₂O₄)。铁氧体 MNPs 呈现出低的磁剩磁，表明在室温下具有超顺磁样响应。然而，不同的磁各向异性和尺寸值导致铁氧体 MNPs 的矫顽力和磁化率值发生变化。通过将 MNPs 内化到 HeLa 癌细胞中来评估不同铁氧体的细胞毒性。尽管磁铁矿和 Mn 铁氧体在所有研究浓度下表现出低毒性，但当用高浓度的 Co 和 Ni 铁氧体孵育细胞时，观察到明显的细胞毒性效应。
