Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo-shi, Chiba 271-8510, Japan.
Department of Tropical Medicine and Parasitology, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Development. 2018 Nov 28;145(23):dev170076. doi: 10.1242/dev.170076.
All flowering plants exhibit a unique type of sexual reproduction called 'double fertilization' in which each pollen tube-delivered sperm cell fuses with an egg and a central cell. Proteins that localize to the plasma membrane of gametes regulate one-to-one gamete pairing and fusion between male and female gametes for successful double fertilization. Here, we have identified a membrane protein from generative cells using proteomic analysis and have found that the protein is an ortholog of DUF679 DOMAIN MEMBRANE PROTEIN 9 (DMP9)/DUO1-ACTIVATED UNKNOWN 2 (DAU2). The flowering plant DMP9 proteins analyzed in this study were predicted to have four transmembrane domains and be specifically expressed in both generative and sperm cells. Knockdown of resulted in aborted seeds due to single fertilization of the central cell. Detailed imaging of -knockdown sperm cells during and semi- double fertilization revealed that DMP9 is involved in gamete interaction that leads to correct double fertilization.
所有开花植物都表现出一种独特的有性生殖方式，称为“双受精”，其中每个花粉管输送的精子细胞与卵子和中央细胞融合。定位于配子质膜的蛋白质调节配子的一对一配对和雌雄配子之间的融合，以实现成功的双受精。在这里，我们使用蛋白质组学分析从生殖细胞中鉴定出一种膜蛋白，并发现该蛋白是 DUF679 结构域膜蛋白 9（DMP9）/DUO1 激活的未知 2（DAU2）的同源物。本研究分析的开花植物 DMP9 蛋白预测具有四个跨膜结构域，并且特异性表达于生殖细胞和精子细胞中。由于中央细胞的单受精，导致 的敲低产生了败育的种子。在 和半双受精过程中对 -敲低的精子细胞进行详细成像显示，DMP9 参与导致正确双受精的配子相互作用。
