Kühn Gianna, Pallauf Kathrin, Schulz Carsten, Birringer Marc, Diaz-Rica Beatriz, de Pascual-Teresa Sonia, Rimbach Gerald
Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.
Institute of Animal Breeding and Husbandry, University of Kiel, Kiel, Germany.
Front Nutr. 2018 Nov 14;5:106. doi: 10.3389/fnut.2018.00106. eCollection 2018.
This study aimed to evaluate whether resveratrol (RSV) and its microbial metabolites dihydro-resveratrol (DHR) and lunularin (LUN) affected fatty acid metabolism and omega-3 polyunsaturated fatty acid (n3-PUFA) synthesis in cultured hepatocytes. To this end, cultured human HepG2 hepatocytes were treated with non-toxic concentrations of these polyphenols (40 μM) and Δ and Δ ( and , respectively) expression was measured. Resveratrol induced both genes but DHR and LUN showed no effect. Co-incubation of RSV with α-linolenic acid (ALA) also induced and expression. Moreover, transcription of and expression was increased, indicating induction of β-oxidation and fatty acid synthesis, respectively. Using gas chromatography to measure fatty acid levels, we observed the impact of RSV with and without ALA treatment on fatty acid composition. However, RSV reduced unsaturated while increasing saturated fatty acid levels. We found lower amounts of monounsaturated fatty acids (16:1n-7c, 18:1n-9c, 18:1n7c, and 20:1n-9) and n3-PUFA docosahexaenoic acid whereas unsaturated fatty acid levels, especially of stearic acid, were elevated. Of interest, once we co-incubated the cells with RSV together with bovine serum albumin, we found no differences in gene expression compared to cells without RSV treatment. Although we found no positive effect of RSV on n3-PUFA synthesis, the stilbene could possibly prevent cellular stress by decreasing unsaturated fatty acid levels.
本研究旨在评估白藜芦醇（RSV）及其微生物代谢产物二氢白藜芦醇（DHR）和月光菌素（LUN）是否会影响培养的肝细胞中的脂肪酸代谢和ω-3多不饱和脂肪酸（n3-PUFA）合成。为此，用无毒浓度的这些多酚（40μM）处理培养的人HepG2肝细胞，并测量Δ和Δ（分别为和）的表达。白藜芦醇诱导了这两个基因，但DHR和LUN没有显示出作用。RSV与α-亚麻酸（ALA）共同孵育也诱导了和的表达。此外，和表达的转录增加，分别表明β-氧化和脂肪酸合成的诱导。使用气相色谱法测量脂肪酸水平，我们观察了有无ALA处理的RSV对脂肪酸组成的影响。然而，RSV降低了不饱和脂肪酸水平，同时增加了饱和脂肪酸水平。我们发现单不饱和脂肪酸（16:1n-7c、18:1n-9c、18:1n7c和20:1n-9）和n3-PUFA二十二碳六烯酸的含量较低，而不饱和脂肪酸水平，尤其是硬脂酸的水平升高。有趣的是，一旦我们将细胞与RSV和牛血清白蛋白共同孵育，我们发现与未用RSV处理的细胞相比，基因表达没有差异。尽管我们没有发现RSV对n3-PUFA合成有积极作用，但这种芪类化合物可能通过降低不饱和脂肪酸水平来预防细胞应激。
