线粒体 PIWI 相互作用 RNA 是透明细胞肾细胞癌的新型生物标志物。
Mitochondrial PIWI-interacting RNAs are novel biomarkers for clear cell renal cell carcinoma.
机构信息
Department of Urology, University Hospital Bonn, Bonn, Germany.
Department of Urology, Second Hospital of Hebei Medical University, Shijiazhuang, China.
出版信息
World J Urol. 2019 Aug;37(8):1639-1647. doi: 10.1007/s00345-018-2575-1. Epub 2018 Nov 28.
PURPOSE
PIWI-interacting RNAs (piRNAs) have been suggested to serve as biomarkers in cancer. In this study, we validated the expression profile of two piRNAs derived from mitochondria, piR-34536 and piR-51810, in tissue and serum of a cohort of clear cell renal cell carcinoma (ccRCC) patients.
METHODS
Tissue and serum samples of patients with ccRCC were collected prospectively in our biobank. Total RNA was isolated from 118 ccRCC tissues, 75 normal renal tissues as well as 30 serum samples from patients with ccRCC, and 15 serum samples from patients with non-malignant diseases. The expression of piRNAs was determined using quantitative real-time PCR.
RESULTS
Both piR-34536 and piR-51810 were downregulated in ccRCC compared to non-malignant renal tissue. Decreased tissue piRNA levels were significant and independent predictors of shortened progression-free, cancer-specific and overall survival of ccRCC patients. The piRNA levels in serum did not differ in ccRCC patients and control subjects.
CONCLUSIONS
The expression of piR-34536 and piR-51810 in ccRCC tissues may be used as prognostic biomarkers in ccRCC.
目的
PIWI 相互作用 RNA（piRNAs）被认为可作为癌症的生物标志物。在这项研究中，我们验证了来自线粒体的两个 piRNA（piR-34536 和 piR-51810）在一组透明细胞肾细胞癌（ccRCC）患者的组织和血清中的表达谱。
方法
前瞻性地在我们的生物库中收集了 ccRCC 患者的组织和血清样本。从 118 例 ccRCC 组织、75 例正常肾组织和 30 例 ccRCC 患者的血清样本以及 15 例非恶性疾病患者的血清样本中分离总 RNA。使用定量实时 PCR 测定 piRNA 的表达。
结果
与非恶性肾组织相比，piR-34536 和 piR-51810 在 ccRCC 中均下调。组织 piRNA 水平降低是 ccRCC 患者无进展生存期、癌症特异性生存期和总生存期缩短的显著且独立的预测因子。ccRCC 患者和对照者的血清 piRNA 水平无差异。
结论
ccRCC 组织中 piR-34536 和 piR-51810 的表达可作为 ccRCC 的预后生物标志物。
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