Sharafutdinova L A, Fedorova A M, Bashkatov S A, Sinel'nikov K N, Valiullin V V
Department of Human Physiology and General Biology, Faculty of Biology, Bashkir State University, Ufa, The Republic of Bashkortostan, Russia.
Department of Histology, Kazan State Medical University, Kazan, The Republic of Tatarstan, Russia.
Bull Exp Biol Med. 2018 Dec;166(2):279-282. doi: 10.1007/s10517-018-4332-2. Epub 2018 Nov 28.
We studied immunohistochemical and morphometric characteristics of the spermatogenic epithelium in rats against the background of peroral administration of nanoparticles containing titanium dioxide. Substantial degenerative changes in the spermatogenic epithelium were revealed: thinning, disorganization of layers, and detachment of sperm cells from the basement membrane. Immunohistochemical analysis revealed reduced proliferative activity and differentiation potential of epithelial cells, which was confirmed by changes in the expression of Ki-67 and c-kit markers. Our data attest to unfavorable effects of titanium dioxide nanoparticles on the structural and functional characteristics of the reproductive system in male rats leading to spermatogenesis disturbances.
我们研究了经口给予含二氧化钛纳米颗粒的大鼠生精上皮的免疫组化和形态计量学特征。结果显示生精上皮出现了明显的退行性变化：变薄、层次紊乱以及精子细胞从基底膜脱离。免疫组化分析显示上皮细胞的增殖活性和分化潜能降低，这通过Ki-67和c-kit标志物表达的变化得到证实。我们的数据证明二氧化钛纳米颗粒对雄性大鼠生殖系统的结构和功能特征产生不利影响，导致精子发生紊乱。
