Al-Musawi Manal M S, Al-Shmgani Hanady, Al-Bairuty Genan A
Biology Department, College of Education for Pure Science/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq.
Department of Microbiology, College of Science, AlKarkh University of Science, Baghdad, Iraq.
Int J Biomater. 2022 May 16;2022:4877637. doi: 10.1155/2022/4877637. eCollection 2022.
Copper (Cu) is an essential trace element for the efficient functioning of living organisms. Cu can enter the body in different ways, and when it surpasses the range of biological tolerance, it can have negative consequences. The use of different nanoparticles, especially metal oxide nanoparticles, is increasingly being expanded in the fields of industry and biomedical materials. However, the impact of these nanoparticles on human health is still not completely elucidated. This comparative study was conducted to evaluate the impacts of copper oxide nanoparticles (CuO NPs) and copper sulphate (CuSO 0.5 (HO)) on infertility and reproductive function in male albino mice BALB/c. Body weight, the weight of male reproductive organs, malondialdehyde (MDA) level, caspase-3 level, and the presence of Ki67 and CD68, as detected using the amino-histochemistry technique, were investigated. Animals were treated with 25 and 35 mg/kg of CuO NPs and CuSO 0.5 (HO) by oral gavage for 14 days. The control group was given distilled water by oral gavage. Body weight significantly decreased at the end of experiments in both treated groups in a concentration- and time-dependent manner compared with the control group. Weights of testes and epididymis (head and tail), as well as the weight of the seminal vesicle, showed a significant decrease compared with the control. However, the average weights of the seminal vesicle and prostate significantly increased. Caspase-3 and MDA levels increased in the CuO NP and CuSO 0.5 (HO) groups compared with the control group, and there was a significant difference between the two concentrations used. Immunohistochemical results detected a significant decrease in Ki67 protein in the treatment groups compared with the control. However, increase in CD68 protein was found in groups treated with CuO NPs and CuSO 0.5 (HO) compared with the control group. Overall, this comparative study of CuO NPs and CuSO 0.5 (HO) showed that oral intake of copper NPs at 25 and 23 mg/kg was safer to the mice reproductive system than CuSO 0.5 (HO) at the same dose. CuSO 0.5 (HO) significantly influenced the histopathological and toxicological alteration responses.
铜（Cu）是生物体高效运作所必需的微量元素。铜可以通过不同方式进入人体，当超过生物耐受范围时，可能会产生负面影响。不同纳米颗粒，尤其是金属氧化物纳米颗粒，在工业和生物医学材料领域的应用日益广泛。然而，这些纳米颗粒对人类健康的影响仍未完全阐明。本比较研究旨在评估氧化铜纳米颗粒（CuO NPs）和硫酸铜（CuSO₄·0.5H₂O）对雄性白化BALB/c小鼠不育和生殖功能的影响。研究了体重、雄性生殖器官重量、丙二醛（MDA）水平、半胱天冬酶 - 3水平，以及使用氨基组织化学技术检测到的Ki67和CD68的存在情况。通过口服灌胃法，给动物分别施用25和35mg/kg的CuO NPs和CuSO₄·0.5H₂O，持续14天。对照组通过口服灌胃给予蒸馏水。与对照组相比，两个处理组在实验结束时体重均以浓度和时间依赖性方式显著下降。睾丸和附睾（头部和尾部）的重量以及精囊的重量与对照组相比显著降低。然而，精囊和前列腺的平均重量显著增加。与对照组相比，CuO NPs和CuSO₄·0.5H₂O组的半胱天冬酶 - 3和MDA水平升高，且所使用的两种浓度之间存在显著差异。免疫组织化学结果显示，与对照组相比，处理组中Ki67蛋白显著减少。然而，与对照组相比，用CuO NPs和CuSO₄·0.5H₂O处理的组中CD68蛋白增加。总体而言，这项对CuO NPs和CuSO₄·0.5H₂O的比较研究表明，以25和35mg/kg口服摄入铜纳米颗粒对小鼠生殖系统的安全性高于相同剂量的CuSO₄·0.5H₂O。CuSO₄·0.5H₂O显著影响组织病理学和毒理学改变反应。
