橙皮苷和百里香酚对变应性鼻炎的改善作用：卵清蛋白诱导的大鼠模型
The amendatory effect of hesperidin and thymol in allergic rhinitis: an ovalbumin-induced rat model.
作者信息
Kilic Korhan, Sakat Muhammed Sedat, Yildirim Serkan, Kandemir Fatih Mehmet, Gozeler Mustafa Sitki, Dortbudak Muhammed Bahaeddin, Kucukler Sefa
机构信息
Department of Otorhinolaryngology, Ataturk University, Faculty of Medicine, 25240, Erzurum, Turkey.
Department of Pathology, Ataturk University, Faculty of Veterinary, Erzurum, Turkey.
出版信息
Eur Arch Otorhinolaryngol. 2019 Feb;276(2):407-415. doi: 10.1007/s00405-018-5222-y. Epub 2018 Nov 28.
PURPOSE
Allergic rhinitis is an immunoglobulin-E (Ig-E)-mediated response driven by type 2 helper T cells. Hesperidin and thymol are biological agents that possess antioxidant and anti-inflammatory characteristics. The purpose of this study was to investigate the effects of hesperidin and thymol in rats with ovalbumin-induced allergic rhinitis.
METHODS
Thirty adult Sprague-Dawley rats were randomly assigned into five groups, each containing six animals. The first group constituted the negative control group, while the remaining groups were exposed to an ovalbumin-induced model of allergic rhinitis. In the provocation stage, 4 mL/kg saline was administered to the positive control group, 10 mg/kg desloratadine to the reference group, 100 mg/kg hesperidin to the hesperidin group, and 20 mg/kg thymol to the thymol group, all by gastric lavage for 7 days. Nasal symptoms were scored on day 22. Rats were then sacrificed, and intracardiac blood specimens were collected to measure plasma total Ig-E, IL-5, IL-13, total antioxidant capacity (TAC), and total oxidant status (TOS) levels. Nasal tissues were extracted for histopathological and immunochemical examination.
RESULTS
Nasal symptom scores were highest in the positive control group, while hesperidin and thymol ameliorated these symptoms to the same extent as desloratadine. Ig-E, IL-5, IL-13, and TOS levels increased, while TAC levels decreased significantly in the allergic rhinitis group compared to the other groups. Significant improvement in these parameters was observed in both the hesperidin and thymol groups. At histopathological and immunohistochemical examination of the nasal cavity, severe allergic inflammation and severe TNF-α expression was determined in rats from the allergic rhinitis group. Mild inflammatory changes and mild TNF-α expression were observed in all three treatment groups.
CONCLUSION
Both hesperidin and thymol were effective in suppressing allergic symptoms and inflammation in the treatment of allergic rhinitis.
目的
变应性鼻炎是由2型辅助性T细胞驱动的免疫球蛋白E（Ig-E）介导的反应。橙皮苷和百里香酚是具有抗氧化和抗炎特性的生物制剂。本研究的目的是探讨橙皮苷和百里香酚对卵清蛋白诱导的变应性鼻炎大鼠的影响。
方法
将30只成年Sprague-Dawley大鼠随机分为五组，每组6只。第一组为阴性对照组，其余各组建立卵清蛋白诱导的变应性鼻炎模型。在激发阶段，阳性对照组经胃灌洗给予4 mL/kg生理盐水，参照组给予10 mg/kg地氯雷他定，橙皮苷组给予100 mg/kg橙皮苷，百里香酚组给予20 mg/kg百里香酚，均持续7天。在第22天对鼻症状进行评分。然后处死大鼠，采集心脏内血液标本以检测血浆总Ig-E、白细胞介素-5（IL-5）、白细胞介素-13（IL-13）、总抗氧化能力（TAC）和总氧化状态（TOS）水平。提取鼻组织进行组织病理学和免疫化学检查。
结果
阳性对照组的鼻症状评分最高，而橙皮苷和百里香酚改善这些症状的程度与地氯雷他定相同。与其他组相比，变应性鼻炎组的Ig-E、IL-5、IL-13和TOS水平升高，而TAC水平显著降低。橙皮苷组和百里香酚组在这些参数上均有显著改善。在鼻腔的组织病理学和免疫组织化学检查中，变应性鼻炎组大鼠出现严重的变应性炎症和严重的肿瘤坏死因子-α（TNF-α）表达。在所有三个治疗组中均观察到轻度炎症变化和轻度TNF-α表达。
结论
橙皮苷和百里香酚在治疗变应性鼻炎时均能有效抑制变应性症状和炎症。
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